应用高通量测序技术分析青海牦牛引进山东后瘤胃菌群的变化

doi:10.16431/j.cnki.1671-7236.2020.07.006

Abstract

Abstract: In order to study the influence of environment and diets on the rumen microflora,the study applied high throughput sequencing technology to analyze the change of Qinghai yak rumen bacterium group after their introduction to Shandong.The results showed that the difference between the Observed species and Chao1 index of Qinghai yak tumor gastric juice and that of Shandong were extremely significant (P<0.01),the ACE index was significantly different(P<0.05).It indicated that bacteria abundance of Qinghai yak rumen microflora was significantly higher than that of Shandong.The shannon index and simpson index showed no significant difference (P>0.05),which indicated that there was no significant change in bacteria diversity of yaks rumen microflora between migration after and before.The bacteria with higher abundance in yak rumen fluid came successively as Bacteroidetes,Proteobacteria,Firmicutes,Verrucomicrobia,Tenericutes,Fibrobacteres.After the migration,Planctomycetes and Armatimonadetes,Oscillibacter and Papillibacter,were among those significantly changed phyla and genera (P<0.05),in terms of composition and proportion.According to the analysis of sequencing,bacteroidetes and Unidentified _Rikenellaceae played an important role in Qinghai yaks rumen liquid,while Prevotellaceae,Succinivibrionaceae and Aeromonadales were crucial part in that of Shandong yaks.Changes in the environment and diet could lead to changes in rumen microflora and related functions.This study provided a theoretical basis for environment and diet affected changes in the yak's rumen microflora structure.

Key words: high-throughput sequencing; yak; rumen microflora; bacteria diversity

CLC Number:

WANG Yunzhou, CHEN Fengmei, ZHANG Wanming, GUO Jianqiang, MA Chaoyin, HU Shilin. High Throughput Sequencing Analyzed Changes in the Yak Rumen Microflora after Qinghai Yaks Introduced to Shandong[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(7): 2013-2024.

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High Throughput Sequencing Analyzed Changes in the Yak Rumen Microflora after Qinghai Yaks Introduced to Shandong

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