中草药泰山磐石散加减方对小鼠肠道微生物菌群的影响

doi:10.16431/j.cnki.1671-7236.2020.08.033

Abstract

Abstract: In order to understand the effects of Chinese herbal medicine of Taishan Panshi san on the intestinal microflora in mice.45 SPF Kunming mice were selected and divided into control group A,test group B,and test group C.The mice in test group B and group C were administered with 0.86 g/mL Chinese herbal medicine of Taishan Panshi san for 0.3 and 0.4 mL respectively,while in group A were administrated with 4 mL distilled water,twice a day for 8 hours for 7 days.On the 8th day,feces samples from the rectum were collected from the control and test groups.Microbial genomic DNA was extracted for each sample.Illumina HiSeq sequencing technology were used to detects sample microbial community diversity.In the aspect of α diversity,the Chinese herbal medicine of Taishan Panshi san showed a fluctuant change in the microbial diversity index (Chao1 and Shannon) in the feces of mice,and the difference between the Chao1 index of the experimental group and the control group was extremely significant (P<0.01);In terms of β diversity,detecting the microorganisms in the feces of control and experimental group of mice,level on the phylum,compared with the control group,there were 9 dominant phylum,Proteobacteria and Verrucomicrobia in the experimental group decreased significantly (P<0.05),while Saccharibacteri and Tenericutes in the experimental group increased significantly (P<0.05;P<0.01);At the family level,there were 19 dominant bacteria families,Lactobacillaceae,Desulfovibrionaceae,Coriobactaceae,Porphyromonaceae,Family_XIII and Biofidobactriaceae in the experimental group were significantly lower than those in the control group (P<0.05);At the genus,there were 18 dominant bacteria,among which,Lactobacillilus,Desulfovibrio and Enterhorhabdus were significantly lower in groups B and C than in group A (P<0.05),while Alloprevotella,Bacteroides,Lachnospiraceae_UCG-006 and Faecalibaculum in the experimental C group increased significantly (P<0.05).The Chinese herbal medicine of Taishan Panshi san could selectively promote the growth of Bacteroidetes in the intestinal tract of mice,thereby significantly inhibiting the harmful effects of Proteobacteria,enhance the metabolism of sugar and fat,finally achieve a new balance of intestinal microbial diversity by improving the structure of intestinal microbial diversity.

Key words: mice; intestinal microflora; Illumina HiSeq sequencing technology

CLC Number:

S859.2

MAI Zhanhai, CUI Yaocheng, SHAYA·Nuerlan, LU Yabin, YAO Weitao, ZHANG Yao, KUANG Ling. Effect of Chinese Herbal Medicine Taishan Panshi San on Intestinal Microbial Community in Mice[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(8): 2634-2642.

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Effect of Chinese Herbal Medicine Taishan Panshi San on Intestinal Microbial Community in Mice

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