三味清热类中药水提物对肉仔鸡肠道菌群和黏膜屏障功能的影响

doi:10.16431/j.cnki.1671-7236.2020.07.009

Abstract

Abstract: In order to study the regulation and repair of intestinal flora and intestinal mucosal damage by single-flavor Chinese medicine,the intestinal flora of broiler chickens caused by Escherichia coli was selected from Chinese herbal medicines of Honeysuckle,Forsythia and Herba violet.It provided a reliable theoretical basis for improving the beneficial flora of intestinal bacteria and protecting intestinal mucosal barrier.100 male broilers of 1 day old were selected and divided into five groups,20 in each group.The blank group was fed normally.The model group and the Chinese medicine group were intraperitoneally injected with E.coli O₇₈ (1.0×10⁶ CFU/mL) at 15-17 days,1 mL each.The Chinese medicine group began to drink traditional Chinese medicine on the 18th day for 7 consecutive days.The dose of each Chinese medicine was 1 g/kg.On the 7 and 14 d after administration,6 chickens in each group were treated with blood stasis.D-lactic acid (D-LA),diamine oxidase (DAO) and endotoxin (ET) were performed according to the Kit instructions by ELISA.Jejunum and cecum were collected.The relative expression levels of jejunal tight junction proteins (ZO-1,Occludin,and Claudin-1) were detected by Real-time fluorescent quantitative PCR.Cecal delivery and detection company was sequenced to detect the influence of traditional Chinese medicine on the diversity of cecal flora.The results showed that Honeysuckle,Forsythia and Herba violet could reduce the levels of serum DAO,D-LA and ET on the 7th and 14th day after administration.On the 7th day after administration,the relative mRNA expression of ZO-1 and Occludin in Honeysuckle group was significantly increased (P<0.05).The relative mRNA expression of Claudin-1 and Occludin in the Forsythia group was significantly increased (P<0.05).On the 14th day after administration,the relative mRNA expression levels of ZO-1 in Honeysuckle group were significantly increased (P<0.05),while the relative mRNA expression levels of ZO-1 and Claudin-1 in Forsythia group and Herba violae were significantly increased (P<0.05).Honeysuckle,Forsythia and Herba violet promoted the proportion of Firmicutes on day 7 after administration (P<0.05),while Honeysuckle,Herba violet promoted the proportion of Bacteroidetes on day 14 after administration (P<0.05).Honeysuckle,Herba violet inhibited the proportion of Proteobacteria at 7 and 14 d after administration,and Herba violet and Forsythia inhibited the growth of Actinobacteria at 7 d after administration (P<0.05).Forsythia,Honeysuckle can significantly increase the proportion of Lactobacillus 7 and 14 d after administration,and Herba violet can significantly increase the proportion of Lactobacillus 7 d after administration (P<0.05).The proportion of the uncultured_bacterium_f_Lachnospiraceae on 14 d after administration was significantly increased by Herba violet.Honeysuckle,Herba violet and Forsythia can reduce the content of serum DAO,D-LA and ET in broilers.Honeysuckle could improve the relative mRNA expression of ZO-1,Occludin and Forsythia could contribute to the relative mRNA expression of ZO-1,Claudin-1 and Occludin.The relative mRNA expression levels of ZO-1 and Claudin-1 were increased by Herba violae.Honeysuckle,Herba violet and Forsythia could increase the number of intestinal OTU,promote the reproduction of beneficial bacteria,inhibit the growth of pathogenic bacteria,and regulate the structure of intestinal flora.

Key words: high-throughput sequencing; heat-clearing traditional Chinese medicine; intestinal flora; testinal mucosal barrier

CLC Number:

S859.3

LIANG Xiaorui, WANG Danyang, QIAO Yanjie, ZHANG Baojun, SUN Guojie, SHAO Yongbin, GU Xinli, LUO Yan. Effects of Water Extract of Three Qingre Chinese Medicine on Intestinal Flora and Mucosal Barrier Function of Broilers[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(7): 2043-2054.

References

[1] XUE M,JI X,LIANG H,et al.The effect of fucoidan on intestinal flora and intestinal barrier function in rats with breast cancer[J].Food & Function,2018,9(2):1214-1223.
[2] BOZENA C,AGNIESZKA S,JOANNA B,et al.Intestinal epithelium,intraepithelial lymphocytes and the gut microbiota-key players in the pathogenesis of celiac disease[J].World Journal of Gastroenterology,2017,23:7505-7518.
[3] XIAO S,LI Q,HU K,et al.Vitamin A and retinoic acid exhibit protective effects on necrotizing enterocolitis by regulating intestinal flora and enhancing the intestinal epithelial barrier[J].Archives of Medical Research,2018,49(1):1-9.
[4] WANG R,ZHOU G,WANG M,et al.The metabolism of polysaccharide from Atractylodes macrocephala koidz and its effect on intestinal microflora[J].Evidence-Based Complementary and Alternative Medicine,2014,2014:926381.
[5] PING Y,MIN C,YAN L,et al.Effects of rhubarb on intestinal flora and toll-like receptors of intestinal mucosa in rats with severe acute pancreatitis[J].Pancreas,2015,44(5):799-804.
[6] YANG L,QIAO Y J,LIU G,et al.Effects of dietary supplementation with leonurine hydrochloride on growth performance,immune response,antioxidant capacity and blood parameters in male broiler chicks[J].Journal of Applied Animal Research,2018,46(1):1490-1495.
[7] 杨春佳,苏德望,王跃生,等.金银花水提物对肠道微生态失调大鼠的调整作用[J].中国微生态学杂志,2012,24(2):132-133. YANG C J,SU D W,WANG Y S,et al.The effect of Chinese medicine double flower on intestinal microflora dysbiosis[J].Chinese Journal of Microecology,2012,24(2):132-133.(in Chinese)
[8] 冉域辰,黎海芪,刘明芳.金银花水提物对双歧杆菌、乳杆菌生长的影响[J].中药药理与临床,2007,5:118-120. RAN Y C,LI H Q,LIU M F.Effects of water extract from Honeysuckle on growth of Bifidobacterium and Lactobacillus[J].Pharmacology and Clinic of Traditional Chinese Medicine,2007,5:118-120.(in Chinese)
[9] 周祎青,郑裕华,陈颂,等.连翘对岭南湿热模型小鼠的作用及其肠道菌群变化的研究[J].中药新药与临床药理,2019,30(6):678-685. ZHOU Y Q,ZHENG Y H,CHEN S,et al.Effects of Forsythiae fructus on Lingnan damp-heat model mice and changes of intestinal flora[J].New Chinese Medicine and Clinical Pharmacology,2019,30(6):678-685.(in Chinese)
[10] 李福娟,崔新刚,王妍,等.紫花地丁水煎剂对小鼠肠道菌群的影响[J].牡丹江医学院学报,2010,31(2):39-40. LI F J,CUI X G,WANG Y,et al.Effects of Herba viole on intestinal flora of mice[J].Journal of Mudanjiang Medical College,2010,31(2):39-40.(in Chinese)
[11] 侯耀杰,姜晓文,李子佳,等.中药复方对大肠杆菌所致肝及肠黏膜损伤修复作用[J].中国兽医杂志,2018,54(3):47-50. HOU Y J,JIANG X W,LI Z J,et al.Repair of liver and intestinal mucosa damage caused by Escherichia coli by traditional Chinese medicine compound[J].Chinese Journal of Veterinary Medicine,2008,54(3):47-50.(in Chinese)
[12] 郭航.二氢杨梅素对肉仔鸡肠黏膜形态及免疫机能的影响[D].杨凌:西北农林科技大学,2008. GUO H.Effects of dihydromyricetin on intestinal mucosal morphology and immune function of broilers[D].Yangling:Northwest A&F University,2008.(in Chinese)
[13] 刘澜,周德刚,莫云,等.26种中草药对奶牛隐性乳房炎病原菌体外抗菌作用的研究[J].中国农业大学学报,2009,14(3):83-88. LIU L,ZHOU D G,MO Y,et al.Antibacterial effects of 26 Chinese herbal medicines on pathogens of dairy cow with subclinical mastitis in vitro[J].Journal of China Agricultural University,2009,14(3):83-88.(in Chinese)
[14] HORIOKA K,TANAKA H,ISOZAKI S,et al.Acute colchicine poisoning causes endotoxemia via the Destruction of intestinal barrier function:The curative effect of endotoxin prevention in a murine model[J].Digestive Diseases and Sciences,2019,10:1007.
[15] AWAD W,HESS C,HESS M.Enteric pathogens and their toxin-induced disruption of the intestinal barrier through alteration of tight junctions in chickens[J].Toxins,2017,9:60.
[16] YANG L,LIU G,LIAN K,et al.Dietary leonurine hydrochloride supplementation attenuates lipopolysaccharide challenge-induced intestinal inflammation and barrier dysfunction by inhibiting the NF-κB/MAPK signaling pathway in broilers[J].Journal of Animal Science,2019,97:1679-1692.
[17] 刘静波,曹山川,杨勇,等.短链果寡糖对断奶仔猪肠道氧化还原状态和屏障功能的影响[J].动物营养学报,2019,31(8):3863-3871. LIU J B,CAO S C,YANG Y,et al.Effects of oligosaccharides on intestinal REDOX status and barrier function of weaned piglets[J].Chinese Journal of Animal Nutrition,2019,31(8):3863-3871.(in Chinese)
[18] SONG M,YE J,ZHANG F,et al.Chenodeoxycholic acid (CDCA) protects against the lipopolysaccharide-induced impairment of the intestinal epithelial barrier function via the FXR-MLCK pathway[J].Journal of Agricultural and Food Chemistry,2019,67:8868-8874.
[19] 呙于明,刘丹,张炳坤.家禽肠道屏障功能及其营养调控[J].动物营养学报,2014,26(10):3091-3100. YU Y M,LIU D,ZHANG B K.Intestinal barrier of poultry:Function and modulation[J].Chinese Journal of Animal Nutrition,2014,26(10):3091-3100.(in Chinese)
[20] 黄登桂,周加义,高春起,等.产肠毒素大肠杆菌对仔猪肠道黏膜屏障功能的影响及其损伤修复研究进展[J].动物营养学报,2019,31(1):48-56. HUANG D G,ZHOU J Y,GAO C Q,et al.Research progress of effects of enterotoxigenic Escherichia coli on intestinal mucosal barrier function and injury repair of piglets[J].Chinese Journal of Animal Nutrition,2019,31(1):48-56.(in Chinese)
[21] CUKROWSKA B,SOWIŃSKA A,BIERŁA B,et al.Intestinal epithelium,intraepithelial lymphocytes and the gut microbiota-key players in the pathogenesis of celiac disease[J].World Journal of Gastroenterology,2017,23:7505-7518.
[22] OH J,PAJARILLO B.Effects of Bacillus subtilis CSL2 on the composition and functional diversity of the faecal microbiota of broiler chickens challenged with Salmonella Gallinarum[J].Journal of Animal Science and Biotechnology,2017,8:1.
[23] 沈起兵,李淑娟,刘同方,等.高通量测序分析鞣花酸对大鼠肠道菌群的影响[J].安徽农业科学,2019,47(18):171-173. SHEN Q B,LI S J,LIU T F,et al.Effects of ellagic acid on gut microbiota in SD rats evaluated by high-throughput sequencing[J].Journal of Anhui Agricultural Science,2019,47(18):171-173.(in Chinese)
[24] 张芹,周中凯,任晓冲.高通量测序技术研究高糖饮食对小鼠肠道菌群的影响[J].食品安全质量检测学报,2015,6(5):1776-1782. ZHANG Q,ZHOU Z K,REN X C.Comparision of intestinal microbiota in mice with normal and high-sugar diet using Miseq high-throughput sequencing[J].Journal of Food Safety and Quality Testing,2015,6(5):1776-1782.(in Chinese)
[25] REIMER R A,MAATHUIS A J,VENEMA K,et al.Effect of the novel polysaccharide PolyGlycopleX^® on short chain fatty acid production in acomputer controlled in vitro model of the human large intestine[J].Nutrients,2014,6(3):1115-1127.
[26] VALENZANO M C,KATHERINE D G,JOANNA M,et al.Remodeling of tight junctions and enhancement of barrier integrity of the CACO-2 intestinal epithelial cell layer by micronutrients[J].PLoS One,2015,10:137.
[27] 石丹,张宇.蒲公英多糖对小鼠肠道微生态的调节作用[J].微生物学免疫学进展,2016,44(3):49-53. SHI D,ZHANG Y.Investigation of regulation from dandelion polysaccharides on mouse intestinal microecology[J].Advances in Microbiology and Immunology,2016,44(3):49-53.(in Chinese)
[28] 张艳冬,陈宇.金银花枝叶提取物对炎症小鼠的抗炎镇痛作用研究[J].浙江中医杂志,2019,54(6):457-458. ZHANG Y D,CHEN Y.Study on the anti-inflammatory and analgesic effects of extracts from branches and leaves of flos ionicerae on inflammatory mice[J].Zhejiang Journal of Traditional Chinese Medicine,2019,54(6):457-458.(in Chinese)
[29] 张静,罗燕,乔彦杰,等.紫花地丁不同提取物化学成分检识及抗炎活性研究[J].石河子大学学报(自然科学版),2019,37(1):121-126. ZHANG J,LUO Y,QIAO Y J,et al.Identification and anti-inflammatory activity of different extracts from viola yedoensis[J].Journal of Shihezi University (Natural Science Edition),2019,37(1):121-126.(in Chinese)
[30] CHENG M,ZHANG X,ZHU J Y,et al.A metagenomics approach to the intestinal microbiome structure and function in high fat diet-induced obesity mice fed with oolong tea polyphenols[J].Food & Function,2018,9:1079-1087.
[31] 王秀武,杜昱光,白雪芳,等.壳寡糖对肉仔鸡肠道主要菌群、小肠微绒毛密度、免疫功能及生产性能的影响[J].动物营养学报,2003,4:32-35. WANG X W,DU Y G,BAI X F,et al.The effects of oligochitosan on broiler gut flora,microvilli density,immune function and growth performance[J].Chinese Journal of Animal Nutrition,2003,4:32-35.(in Chinese)
[32] 彭骞骞,王雪敏,张敏红,等.持续偏热环境对肉鸡盲肠菌群多样性的影响[J].中国农业科学,2016,49(1):186-194. PENG Q Q,WANG X M,ZHANG M H,et al.Effects of constant moderate temperatures on the diversity of the intestinal microbial flora of broilers[J].Scientia Agricultura Sinica,2016,49(1):186-194.(in Chinese)
[33] 龚梦鹃,李春苑,巫圣乾,等.16S rRNA高通量测序研究藿香正气口服液对湿困脾胃证大鼠肠道菌群的影响[J].中药新药与临床药理,2019,30(4):391-395. GONG M J,LI C Y,WU S Q,et al.Effects of Huoxiang Zhengqi oral liquid on gut microbiota in rats with dampness obstructing spleen-stomach syndrome based on high-throughput 16S rRNA gene sequencing[J].Chinese Medicine and Clinical Pharmacology,2019,30(4):391-395.(in Chinese)

Effects of Water Extract of Three Qingre Chinese Medicine on Intestinal Flora and Mucosal Barrier Function of Broilers

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