发酵中药对蛋鸡生长性能、抗体水平及肠道菌群的影响

doi:10.16431/j.cnki.1671-7236.2022.06.015

Abstract

Abstract: 【Objective】 The purpose of this experiment was to study the effects of fermented traditional Chinese medicinal on the growth performance, antibody level and intestinal flora of layer hens.【Method】 84 1 day old Cobb laying hens were selected and pre fed to 13 days old.The formal experiment was conducted at 14 days old.The chickens were randomly divided into 7 groups, each group was raised in a single cage, with 12 per cage.They were blank control group (group A), compound traditional Chinese medicine group (group B), low-dose fermented traditional Chinese medicine group (group C), middle-dose fermented traditional Chinese medicine group (group D), high-dose fermented traditional Chinese medicine group (group E), fermentation broth group (group F) and control drug Astragalus polysaccharide group (group G).The daily feed and leftover was recorded at each stage during the test.The chicks were weighed on an empty stomach in the early morning on the 1st (14 days of age), 14th (28 days of age) and 28th (42 days of age) days, and the average daily gain (ADG), average daily feed intake (ADFI) and feed-to-gain ratio (F/G) were calculated at each stage.On the 7th, 14th, 21st and 28th days of the experiment, blood was collected from the veins of chicken wings in each group for the detection of antibody titer.At the end of the experiment, the chickens of groups A, B, D, F and G were subjected to necropsy, the cecal contents of 5 chickens in each group were subjected to high-throughput sequencing analysis.【Result】 During the 1st to 14th day, the ADFI of groups D and E were extremely significantly higher than that of groups A, F and G (P<0.01), the ADG of group E and D were extremely significantly or significantly higher than that of groups A and F (P<0.01 or P<0.05).The F/G of groups F and G were extremely significantly lower than that of other groups (P<0.01).During the 15th to 28th day, the ADFI of groups C and E were significantly higher than that of groups B and F (P<0.05), the ADG of group B was extremely significantly higher than that of other groups (P<0.01), and the ADG of group E was significantly higher than that of group A (P<0.05).The F/G of group B was extremely significantly lower than that of other groups, and the F/G of groups D, E, F and G were extremely significantly lower than that of group A (P<0.01).On the 7th day, there was no significant difference of serum antibody titer in chickens in each group (P>0.05).On the 14th day, the antibody level of group E was significantly higher than that of groups A, F and G (P<0.05).On the 21st and 28th days, the antibody level of group E was significantly higher than taht of group A (P<0.05).Group D had the highest Chao1 and Observed species indexes, but had no significant relationship with the other groups (P>0.05).At the phylum level, the relative abundance of Bacteroidetes in cecum of groups D and G were significantly higher than that of group A (P<0.05).At the genus level, the relative abundance of Bacteroides in cecum of group D was significantly higher than that of group A (P<0.05).【Conclusion】 Fermented traditional Chinese medicine could improve the growth performance and antibody level of chickens, and it also had a significant regulatory effect on the intestinal flora.

Key words: fermented traditional Chinese medicine; layer hens; growth performance; antibody titer; intestinal flora

CLC Number:

S831.4

SHI Jiancun, LI Jikai, JIA Lin, LYU Jiancun, SHANG Zihan, ZHANG Tie, WANG Chunguang. Effects of Fermented Traditional Chinese Medicine on the Growth Performance, Antibody Level and Intestinal Flora of Laying Hens[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2145-2155.

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Effects of Fermented Traditional Chinese Medicine on the Growth Performance, Antibody Level and Intestinal Flora of Laying Hens

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