杂交鲟类志贺邻单胞菌的分离鉴定及耐药性分析

doi:10.16431/j.cnki.1671-7236.2019.01.031

Abstract

Abstract:

This paper was aimed to study on isolation,identification and drug resistance analysis of a bacterial (NZ-2017),which was isolated from local sick hybrid sturgeons.Morphology,physiological and biochemical characteristics and phylogenetic analysis of 16S rRNA gene were used to identify its species.The filter paper diffusion method test,antimicrobial resistance genes test and animal regression test were carried out to analyze its pathogenicity.The results showed that NZ-2017 was a gram-negative bacillus,which was 99.70% homologous with Plesiomonas shigelloides 16S rRNA gene fragment by sequencing and BLAST analysis.With the results of physiological and biochemical identification,NZ-2017 was confirmed as Plesiomonas shigelloides.The drug sensitivity test results showed that NZ-2017 was sensitive to ofloxacin,norfloxacin,ciprofloxacin,doxycycline,and resistance to kanamycin,amoxicillin,tylosin and sulfamethoxazole.The antimicrobial resistance genes test results showed that there were three antimicrobial resistance genes (Sul1,Sul2 and Intl1),which was compatible with phenotype.The animal regression test result showed that NZ-2017 had pathogenicity to mouse.This study conducted an accurate diagnosis of the cause of the epidemic of sturgeons in Guizhou,and provided a basis for the prevention and controls to this bacterial disease of fish in this region.

Key words: hybrid sturgeon; Plesiomonas shigelloides; isolation and identification; 16S rRNA gene; antibiotic sensitivity test; antimicrobial resistance genes

CLC Number:

S852.61⁺2

ZHANG Mingyang, HU Andong, CHENG Zhentao, JIANG Haibo, WEN Ming. Isolation,Identification and Drug Resistance Analysis of Plesiomonas shigelloides from Hybrid Surgeon[J]. , 2019, 46(1): 264-270.

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Isolation,Identification and Drug Resistance Analysis of Plesiomonas shigelloides from Hybrid Surgeon

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