广西猪源产肠毒素大肠杆菌毒力因子及多重耐药性分析

doi:10.16431/j.cnki.1671-7236.2019.03.033

Abstract

Abstract:

This study was aimed to investigate the possible pathogens and the main causes of disease in a pig farm in Guangxi.The bacteria were isolated and identified by bacterial separation and purification,morphological identification,growth curve measurement,biochemical analysis and drug susceptibility test.The strain 16S rRNA,virulence genes and drug resistance genes were detected by PCR.The results showed that the strain isolated from the disease material was a Gram-negative bacterium with no spores,pili,and round and short rods at both ends,which had strong reproductive ability and rapid growth,and was suspected to be Escherichia coli (E.coli).The 16S rRNA amplification results showed that a 1 474 bp band appeared,and the sequencing results showed that the homology of the isolate with E.coli was 99%.The strain showed strong resistance to 31 antibacterial drugs gentamicin,tilmicosin,tetracycline,enrofloxacin,penicillin,lincomycin,sulfamethoxazole,etc.,all showed higher levels of drug resistance,and the drug resistance rate was as high as 87.10% (27/32).It was only sensitive to two drugs,amikacin and colistin.Toxicity factor detection identified a total of five virulence factors,FyuA,enterotoxin STb and LT,adhesin F41 and K88.The detection rate was 35.70% (5/14).It had been amplified β-lactamases bla_TEM and bla_OXA,tetracycline tet(A),sulfonamide Sul2,aminoglycosides aadA1 and aac(3')-Ⅱa,quinolone GyrA,GyrB and ParC,amide alcohol floR 10 drug resistance genes,the detection rate was 66.70% (10/15),and the detection of drug resistance genes was positively correlated with drug resistance phenotype.In summary,the strain presented a variety of complex virulence factors,complex drug-resistance,and serious multi-drug resistance,and corresponding precautions need to be taken to prevent spread and spread.

Key words: enterotoxin production; multi-drug resistance; Escherichia coli; virulence factor

CLC Number:

S852.61

GE Chenling, SHI Dali, HU Wen, SONG Xingxing, GE Qiang, MO Yupeng, HU Chuanhuo, WANG Xiaoye. Analysis of Virulence Factors and Multidrug Resistance of Enterotoxin-producing Escherichia coli in Guangxi[J]. , 2019, 46(3): 913-923.

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Analysis of Virulence Factors and Multidrug Resistance of Enterotoxin-producing Escherichia coli in Guangxi

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