纳米银对猪链球菌体外抗菌活性及其生物膜形成的影响

doi:10.16431/j.cnki.1671-7236.2022.03.040

Abstract

Abstract: 【Objective】 The effects of silver nanoparticles (AgNPs) on the in vitro antibacterial activity and biofilm formation of multidrug resistant Streptococcus suis were studied to provide reference for solving the drug resistance problem of Streptococcus suis.【Method】 Five clinically isolated multi-drug resistant Streptococcus suis (S1018, S894, S786, S815 and S844) were used as the research objects.The minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) of AgNPs against Streptococcus suis were determined by micro broth dilution method.The antibacterial activity of AgNPs was evaluated by measuring the growth rate of Streptococcus suis under the action of AgNPs and the inhibition rate of different concentrations of AgNPs on Streptococcus suis.Furthermore, the biofilm formation ability of each strain and the effect of AgNPs with concentrations of 6.25, 12.5, 25 and 50 μg/mL on biofilm formation of 5 multi-drug resistant Streptococcus suis strains were evaluated by crystal violet staining.【Result】 The MIC and MBC of AgNPs against S1018 strain were both 12.5 μg/mL, and the MIC and MBC of AgNPs against the other four multi-drug resistant Streptococcus suis strains were all 25.0 μg/mL.AgNPs had good antibacterial activity against multi-drug resistant Streptococcus suis.The inhibition rate of AgNPs on 5 strains of Streptococcus suis could reach 96.60%-99.70% at the concentration of 25-100 μg/mL, and the growth rate of bacteria was significantly inhibited under the action of 1/2MIC, 1MIC and 2MIC AgNPs.5 strains of multi-drug resistant Streptococcus suis could form biofilms, and the inhibition rates of AgNPs at concentrations of 6.25, 12.5, 25, 50 μg/mL on their biofilm formation were 17.98%-45.58%, respectively.【Conclusion】 AgNPs inhibited the activity and biofilm formation of multi-drug resistant Streptococcus suis.

Key words: silver nanoparticles; multi-drug resistance; Streptococcus suis; biofilm

CLC Number:

S852.61

LIU Baoling, LI Chunling, JIANG Zhiyong, YANG Dongxia, ZHANG Kunli, CHU Pinpin, GOU Hongchao, CAI Rujian. Effects of Silver Nanparticles on the Activity of Streptococcus suis and Its Biofilm Formation in vitro[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 1172-1180.

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Effects of Silver Nanparticles on the Activity of Streptococcus suis and Its Biofilm Formation in vitro

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