广西某猪场猪链球菌分离鉴定及其生物学特性研究

doi:10.16431/j.cnki.1671-7236.2021.02.036

Abstract

Abstract: In December 2019,a series of sudden deaths of protected pigs occurred in a swine farm in Yulin,Guangxi.In order to confirm the cause of death and investigate the pathogenicity and drug resistance of isolated strains,samples and bacterial isolation and culture were conducted in the sick swine farm.Morphological observation,biochemical identification,PCR amplification of gdh gene,identification of serum type and some virulence genes,detection of drug resistance and identification of drug resistance genes were performed on the isolates.One bacterial strain was isolated from two dead pigs separately.On TSB solid medium,the colonies were gray white,smooth,and uniform in size.They were preliminarily identified as Gram-positive Streptococcus and named GXYL-F1 and GXYL-N2,respectively.Both isolates were identified as Streptococcus suis type 9 by specific gene gdh and serum primer.Biochemical identification results showed that the two isolated strains could ferment salicylline,maltose,M.R.and aescinate.The virulence gene of the strain detected by PCR showed that genes gdh,fbps,sao,sbp2' and orf2 were positive,while mrp,epf and sly genes were negative.Drug sensitivity test results showed that GXYL-F1 and GXYL-N2 were all resistant to neomycin,streptomycin,timicoxacin,tylosin,erythromycin,tetracycline,levofloxacin,penicillin and sulfadiazine.In addition,the isolated strain GXYl-F1 was also resistant to enrofloxacin.The drug-resistant gene aadA1,qnrB and qnrS were amplified in both isolates.The study showed that the disease was caused by Streptococcus suis type 9.The above research results provided a reference for this swine farm to develop the control measures of Streptococcus suis type 9 and a new data for the serotype diversity and prevention of technology reaserch.

Key words: swine; Streptococcus suis; PCR identification; drug resistance; virulence gene; drug sensitive test

CLC Number:

S852.61

GUO Changming, CHEN Huaijun, ZHU Shanyuan, YUAN Jingzhi, GE Qiang, LI Xun, WANG Xiaoye. Isolation and Identification of Streptococcus suis and Biological Characteristics Research on a Swine Farm in Guangxi[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(2): 736-746.

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Isolation and Identification of Streptococcus suis and Biological Characteristics Research on a Swine Farm in Guangxi

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