猪β防御素-2对猪源肠外致病性大肠杆菌的体内外抑菌活性研究

doi:10.16431/j.cnki.1671-7236.2021.02.035

Abstract

Abstract: The purpose of this study was to evaluate the antibacterial activity of porcine beta defensin 2 (PBD-2) against porcine extraintestinal pathogenic Escherichia coli (ExPEC) in vivo and in vitro,and provide more insights for evaluating the value of PBD-2 as an antibiotic substitutes.Firstly,the bactericidal activity of different concentrations of PBD-2 against the porcine ExPEC PCN033 was tested in vitro.Subsequently,female Kunming mice aged 5 weeks and weighing between 18-22 g infected with different doses of ExPEC PCN033 were treated with PBD-2 or PBS (n ≥ 5).The survival rate,the bacteria loads of brain,spleen,lung tissue and blood,the levels of inflammatory cytokines IL-6,IL-12,IL-1β and TNF-α and the pathological changes of brain,spleen and lung tissues were observed.The results showed that 25 μg/mL of PBD-2 could extremely significantly inhibit the growth of ExPEC PCN033 in a concentration-dependent manner (P<0.01).The in vivo study revealed that PBD-2 treatment could effectively reduce the mortality rate of mice infected with ExPEC PCN033.The effects of PBD-2 were enhanced with increase of treatment dose of PBD-2.The effects of PBD-2 via intraperitoneal injection and intramuscular injection route were better than that of the oral route.PBD-2 treatment was less effective than chloramphenicol treatment in reducing the mortality of mice.At the same time,PBD-2 treatment extremely significantly reduced the bacteria loads in the brain,spleen,lung tissue and blood of mice at 21 h post infection with ExPEC PCN033 (P<0.01).At the same time,PBD-2 treatment extremely significantly reduced the levels of IL-6,IL-12 and IL-1β of mice at 21 h post infection with PCN033 (P<0.01).PBD-2 treatment also significantly reduced the degree of pathological damages of the brain,lung and spleen tissues of mice at 21 h post infection with ExPEC PCN033.These results indicated that PBD-2 had good activity against porcine ExPEC in vitro,and had therapeutic effect on swine derived ExPEC infection in mice,indicating that PBD-2 had the potential to develop into therapeutic drugs or antibiotic substitutes.

Key words: porcine beta defensin 2 (PBD-2); bacterial infection; treatment; extraintestinal pathogenic Escherichia coli (ExPEC)

CLC Number:

S855.1

WANG Antian, HUANG Jing, SUN Yufan, SONG Bingxiao, TAN Chen, HUANG Qi, ZHOU Rui, LI Lu. Antibacterial Activity of Porcine Beta Defensin-2 Against Porcine Extraintestinal Pathogenic Escherichia coli in vivo and in vitro[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(2): 726-735.

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Antibacterial Activity of Porcine Beta Defensin-2 Against Porcine Extraintestinal Pathogenic Escherichia coli in vivo and in vitro

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