单碱基编辑技术的应用研究进展

doi:10.16431/j.cnki.1671-7236.2021.12.008

Abstract

Abstract: Single base editor (BE) is a new generation of gene editing technology based on CRISPR/Cas9.Compared with the traditional gene editing technology, single base editing has the unique advantages of high efficiency, safety and less by-products.It is gradually replacing the traditional techniques such as zinc finger nuclease (ZFN) technology, transcription activator-like effector nuclease (TALEN) technology, clustered regularly interspaced short palindromic repeats/Cas (CRISPR/Cas) and become the mainstream gene editing technology.Single base editing technology has greatly promoted the development of livestock gene editing and human disease treatment and other research fields.With the continuous optimization of deaminase and Cas nuclease, the accuracy of single base editing is improving and the targeting range is expanding, so that the technology can be more widely used in the field of life science.The advantages and disadvantages of previous gene editing technology were compared.The main function structure, editing principle and optimization process of single base editing technology were reviewed.The advantages and disadvantages of single base editing technology were analyzed.This review focuses on the application of single base editing technology in the improvement of important economic traits such as disease resistance, meat yield, wool yield and fecundity of livestock and the treatment of human gene diseases.In this review, the further application of single base editing technology in improving multiple economic traits of livestock and overcoming the problem of pig gamete cryopreservation was proposed.

Key words: single base editing; CRISPR/Cas9; livestock breeding; disease treatment

CLC Number:

XU Xi, YANG Sha, HAO Haisheng, DU Weihua, PANG Yunwei, ZHAO Shanjiang, ZOU Huiying, ZHU Huabin, LI Shujing, YU Wenli, ZHAO Xueming. Research Progress on Application of Single Base Editing Technology[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(12): 4403-4411.

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Research Progress on Application of Single Base Editing Technology

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