Abstract: Veterinary sulfonamides are often added to animal foods to treat disease and promote growth,however,there is a risk of SAs residues remaining in animal products if these drugs have been administered improperly.Abusing of sulfonamides has generated potentially serious problems in human health and affected food safety.In addition,sulfonamides cannot be completely absorbed in humans and animals and most of them are excreted in urine and feces in the form of prototypes and metabolites,causing a series of problems such as bacterial drug resistance.Therefore,it is necessary to study the residues of sulfonamides in animal products and environment for ensuring food safety and protecting people's health.Currently,chromatography and mass spectrometry are the main methods for the detection of sulfonamides and their metabolites.Pretreatment is necessary in residue detection because of the complex sample matrix and low drug concentrations.In recent years,the sample preparation is developing towards miniaturization,solvent-free and automation.The authors introduced the principles,advantages,disadvantages and application of various sample pretreatment technologies including the solid phase extraction,solid phase microextraction,dispersed liquid phase microextraction,hollow fiber-based liquid-phase microextraction,matrix solid-phase dispersion,molecular imprinting polymers,immune affinity chromatography,field assisted extraction,QuEChERS and cloud point extraction and made outlook pretreatment for the future development direction.

Key words: sulfonamides; metabolites; animal food; environment; pretreatment technique