甘肃某地区奶牛运动场环境中耐药菌流行情况及相关耐药基因检测

doi:10.16431/j.cnki.1671-7236.2020.06.028

Abstract

Abstract: In order to investigate the distribution of major resistant bacteria and the resistant genes around the environment of dairy cow pastureland in Gansu province,the resistant bacteria were isolated and purified by resistance plate screening method,and then identified by 16S rRNA in the study.The results showed that a total of 665 strains of different species of resistant bacteria were isolated from the two fields (farm A and farm B).The major strains were Escherichia coli,Enterococcus faecalis and Proteus genera by PCR identification of 16S rRNA.The number of resistant Escherichia coli was the largest (398 strains).The number of resistant bacteria in farm A increased by 28.50% over the past two years,while the number of resistant bacteria in farm B remained the same.The results of 10 kinds of resistant bacteria screening showed that the isolation rates of the first three categories were glycopeptide (11.13%,74/665),tetracycline and sulfonamide (10.98%,73/665),and beta-lactam and aminoglycoside (10.23%,68/665).PCR results showed that the resistant genes in dairy cow pastureland during two years period of time were almost similar.The prevalence rates of quinolone resistant genes qnrB and qnrS were 10.81% and 83.78%,respectively.The prevalence rates of sulfanilamide resistant genes Sul1,Sul2 and Sul3 were 100%,100% and 56.76%,respectively.The prevalence rates of glycopeptide resistance genes VanA,VanB and VanC were 78.38%,100% and 100%,respectively.The prevalence rates of tetracycline resistant genes tetA,tetB, tetC,tetM,tetO and tetL were 100%,100%,100%,100%,81.08% and 75.68%,respectively.The prevalence rates of KPC,NDM and aac(6')-Ⅰb -cr resistance genes were 32.43%,29.73% and 24.32%,respectively.However,qnrC,tetK,tetW,VIM and OXA resistance genes were not detected.The homology between resistance gene and the gene sequence of reference strain in GenBank was 98% to 99%.The resistant bacteria carrying corresponding resistance gene was very common in Gansu province.Especially,carbapenems and aminoglycoside resistance genes were detected in high number.Therefore,regular surveillance of drug-resistant bacteria was highly essential in order to minimize the risk caused by resistant bacteria to human.In addition,antibiotic drugs should be used wisely and in appropriate dose during clinical treatment.

Key words: dairy cow; pastureland environment; resistant bacteria; isolation and identification; resistence gene

CLC Number:

S852.61

ZHANG Xuejing, HE Zhuolin, HOU Xiao, PU Wanxia. Detection of Resistance and Resistance Genes of Dairy Cow Pastureland Environment in Gansu Province[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(6): 1882-1891.

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Detection of Resistance and Resistance Genes of Dairy Cow Pastureland Environment in Gansu Province

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