环境高温对通城猪血清氧化应激因子及空肠黏膜机械屏障功能的影响

doi:10.16431/j.cnki.1671-7236.2020.10.009

Abstract

Abstract: The study was aimed to investigate the effects of high temperature on serum oxidative stress indexes and jejunal mucosal mechanical barrier function in Tongcheng pigs.Thirty 100-day-old female Tongcheng pigs with similar genetic backgrounds and average initial body weight of 45 kg±5 kg were randomly divided into six groups.All pigs were firstly raised under (28±1) ℃ with ad libitum feeding for 7 d.Then the control group was still raised under (28±1) ℃,the high temperature groups were treated with heat preservation lamps,and the temperature was rapidly raised to (38±1) ℃.Pigs were slaughtered and samples were collected at 2,4,6,8,and 10 days,respectively.Serum oxidative stress indexes and the expression levels of mechanical barrier and inflammatory factor-related mRNA in jejunum mucosa were detected.HE sections and immunohistochemical staining were performed on jejunum tissues.The results showed that ①Compared with the control group,the content of serum MDA and GSH-Px activity decreased significantly on the 2nd day of high temperature (P<0.05),and then stabilized.There was no significant change in serum SOD in high temperature groups (P>0.05).Serum T-AOC increased significantly on the 2nd and 8th day of heat treatment (P<0.05).②Compared with the control group,the expression of tight junction proteins ZO-1,OCLN,CLDN5 and CLDN7 mRNA decreased significantly (P<0.05),while the expression of cytoskeleton proteins IQGAP1,IQGAP2 and IQGAP3 mRNA decreased significantly (P<0.05),and the expression of Rac1 mRNA decreased significantly (P<0.05),the expression of Rac2 and Rac3 mRNA did not change significantly (P>0.05) in the heat stress group (2 days).Compared with the control group,the expression of HSP70 in jejunal mucosa increased,mucosal structure was damaged and inflammation occurred in heat stress group.In conclusion,Tongcheng pigs had good antioxidant stress regulation ability under high temperature environment,but high temperature would weaken jejunal barrier function.

Key words: high temperature environment; Tongcheng pigs; oxidative stress; jejunum; mechanical barrier; inflammation

CLC Number:

Q494

GAO Dengying, LEI Minggang. Effects of High Environmental Temperature on Serum Oxidative Stress Factors and Jejunal Mucosal Mechanical Barrier Function in Tongcheng Pigs[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(10): 3114-3122.

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Effects of High Environmental Temperature on Serum Oxidative Stress Factors and Jejunal Mucosal Mechanical Barrier Function in Tongcheng Pigs

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