合成绵羊抗菌肽NK-Lysin抗菌活性及其对雏鸡沙门氏菌攻毒的治疗效果研究

doi:10.16431/j.cnki.1671-7236.2017.09.028

Abstract

Abstract:

In order to study the biological function of the main active region of sheep antimicrobial peptide NK-Lysin, three pairs of functional domain peptide fragments were designed and synthesized the antibacterial activity was detected by radial diffusion test and minimal inhibitory concentration. We analyzed the toxic effects of chicken red blood cells and screen the best peptides for treatment chicken challenged by Salmonella pullorum in this study. The rusults showed that synthesized peptides were inhibitory to Escherichia coli and Salmonella pullorum. Fragment length of peptides, C-terminal amidation and peptide inner loop were essential to antibacterial activity. Two of synthesized peptides were used to treat chichen challenged by Salmonella pullorum which obviously decreased the mortality of chicken. The pathological damage of heart,liver,kidney were less than that of control group. This study laid a foundation for the development of the sheep NK-Lysin peptides as candidate antimicrobial agents.

Key words: sheep; antimicrobial peptide; Salmonella; challenge; treatment

CLC Number:

S852

YANG Xia, ZHANG Dian-qing, SONG Jia-wei, XIAO Sheng-zhong, SHENG Jin-liang, YAN Gen-qiang. Antimicrobial Activity of Synthesized Sheep NK-Lysin Peptides and Its Treatment for Chicken Salmonella Challenge[J]. , 2017, 44(9): 2739-2746.

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Antimicrobial Activity of Synthesized Sheep NK-Lysin Peptides and Its Treatment for Chicken Salmonella Challenge

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