猪链球菌14型的分离鉴定及生物学特性研究

doi:10.16431/j.cnki.1671-7236.2020.06.026

Abstract

Abstract: In order to detect and determine the pathogen of the diseased piglets in a large-scale pig farm in Henan province in May 2019,a strain of bacteria was isolated from the joint fluid of diseased piglets.The isolated strain was identified as Streptococcus suis by purification culture,Gram staining,morphological observation and PCR amplification of gdh gene of Streptococcus suis.PCR amplification and software were used to identify the Streptococcus suis.The results showed that the strain was Streptococcus suis serotype 14 and 100% homologous with js14 (GenBank No.:CP002465.1).The results of virulence gene detection showed that epf,mrp,sly,fbps and orf2 virulence genes were carried by the strain at the same time.The results of mouse pathogenicity test also showed that the strain was a highly pathogenic Streptococcus suis.The results of drug sensitivity test showed that the strain was sensitive to β-lactams and quinolones,and highly resistant to aminoglycosides,tetracyclines,macrolides and sulfonamides,showing multiple drug resistance.The resistance genes of bla_TEM,aadA1,strA,strB,aacC2,aphA1,tet(B),gyrA,parC and sul2 were detected.This study laid a foundation for further research on the epidemic characteristics and pathogenic mechanism of Streptococcus suis serotype 14,provided theoretical basis for clinical prevention and control of Streptococcus suis serotype 14,and had important public health significance.

Key words: Streptococcus suis serotype 14; typing and identification; virulence gene; drug sensitivity test; drug resistance gene; pigs

CLC Number:

S852.61

WANG Zhifang, XU Yindi, ZHANG Qingxian, ZHU Wenhao, BAI Hongjie, JIAO Wenqiang, LI Haili, XU Feng, WANG Keling, ZHANG Bin, LOU Zhiguo. Isolation,Identification and Biological Characteristics of Streptococcus suis Serotype 14[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(6): 1861-1869.

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Isolation,Identification and Biological Characteristics of Streptococcus suis Serotype 14

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