天津地区猪链球菌3型菌株的致病性及耐药特性研究

doi:10.16431/j.cnki.1671-7236.2019.12.034

Abstract

Abstract: The aim of this study was to investigate the pathogenicity and drug resistance of 7 Streptococcus suis type 3 (SS 3) strains isolated from piggery in Tianjin.The pathogenicity of bacteria with doses of 1.0×10⁷,1.0×10⁸ and 1.0×10⁹ CFU/mice was studied.The virulence genes mrp,ef,sly,gdh,gapdh,fbps and orf2 of 7 strains of SS 3 strains were detected by PCR.Drug susceptibility tests of the 7 strains of SS 3 were also carried out.The results of pathogenicity test showed that 6 and 3 strains of SS 3 could cause 100.0% (5/5) morbidity and death in mice at inoculation dose of 1.0×10⁹ and 1.0×10⁸ CFU/mice,respectively.One strain of SS 3 could still cause 80.0% (4/5) morbidity and death in mice at inoculation dose of 1.0×10⁷ CFU/mice.The order of pathogenicity of the 7 strains of SS3 strains in mice was R15056 > R15042=S15030 > Y12024=Y09011 > Y13125 > Y13164.The results of virulence gene test showed that there were three virulence genotypes:R12024,Y13164,R15042 and S15030 were positive for gdh,gapdh,fbps and orf2 virulence genes;Y13125 and R15056 were positive for sly,gdh,gapdh,fbps and orf2 virulence genes,and Y09011 was positive for gdh,fbps and orf2 virulence genes.Drug susceptibility test showed that 7 SS 3 strains were relatively sensitive to cefquinoxime,followed by amoxicillin,ciprofloxacin and sulfamethoxacil sodium.However,they showed 100.0% resistance to doxycycline,and all strains showed more than 3-fold resistance.This study laid a foundation for the further development of epidemiological characteristics and pathogenic mechanism of SS 3 strains in Tianjin,and could provide theoretical guidance for the comprehensive prevention and control of SS 3 strains in Tianjin.

Key words: Streptococcus suis type 3; identification; pathogenicity; virulence gene; drug sensitivity test

CLC Number:

S852.61⁺1

CHI Jingjing, DONG Zhimin, TIAN Xiangxue, TAN Shixu, LI Xiuli, YANG Chunlei, REN Weike, YAN Minghua. Study on Pathogenicity and Drug Resistance of Streptococcus suis Type 3 Strain in Tianjin[J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(12): 3740-3748.

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Study on Pathogenicity and Drug Resistance of Streptococcus suis Type 3 Strain in Tianjin

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