有机农场鸡、猪源大肠杆菌耐药性研究

doi:10.16431/j.cnki.1671-7236.2015.12.035

Abstract

Abstract: To determine the drug resistance of E.coli strains from swine and chicken in organic farm,collection of stool specimens was conducted in one organic farm in Conghua city,Guangdong province in 2014,in which 118 E.coli strains were separated,including 55 from chicken and 63 from swine.The sensitivity of the E.coli strains from swine and chicken to 18 kinds of drugs was detected through agar dilution method.Similar properties in drug resistance were observed both in E.coli strains from swine and chicken in the same organic farm.In consideration of the resistance rate,samples were relatively sensitive to ceftazidime,cefquinome,cefoxitin,amikacin,apramycin,gentamicin,neomycin,florfenicol,chloramphenicol,imipenem,ciprofloxacin and olaquindox (chicken 0 to 21.8%;swine 0 to 14.3%).These isolates showed moderate resistance to ampicillin and streptomycin (chicken 29.1% to 38.2%;swine 23.8% to 27.0%).About 56.4% chicken isolates and 47.6% swine isolates showed resistant to more than 3 kinds of drugs.However,severe resistance was observed in trimethoprim-sulphamethoxazole,tetracyclineand and doxycycline (chicken 45.5%,76.4% and 72.7%;swine 50.8%,81.0% and 68.3%).The results also indicated that under organic farming mode,the drug resistance rates of E.coli strains from swine and chicken were relatively low in general.Multi-drug resistance existed but was relatively slight.Although the drug resistance rate to several specific antimicrobials was high,it was still lower than that in conventional farms.Since most studies focused on the drug resistance of the bacteria under conventional farming model,this study filled the blank of the research of drug resistance under organic farming model.

Key words: organic farm; Escherichia coli; drug resistance; multi-drug resistance

CLC Number:

S852.61⁺4

ZHI Chan-ping, GUO Xiao-ting, YAO Xu, GUO Ze-wen, WU Sheng-jun, YI Ling-xian, CHENG Bang-zhao, WANG Jun-jun, LIU Jian-hua. Study on Drug Resistance of Escherichia coli Isolated from Chicken and Swine in Organic Farm[J]. , 2015, 42(12): 3351-3357.

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Study on Drug Resistance of Escherichia coli Isolated from Chicken and Swine in Organic Farm

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