口蹄疫疫苗非抗原蛋白对146S抗原免疫效果的影响

doi:10.16431/j.cnki.1671-7236.2019.03.032

Abstract

Abstract:

In order to explore the effect of non-antigen protein on the immune effect of foot-and-mouth disease virus type A (FMDV-A) 146S antigen,mice and pigs were used as experimental animals.5 groups of mice for injection were prepared:Group A (3 μg 146S antigen),group B (3 μg 146S antigen＋25 μg non-antigenic protein),group C (3 μg 146S antigen＋50 μg non-antigenic protein),group D (3 μg 146S antigen＋100 μg non-antigenic protein) and group E (blank control,PBS).At the same time,four groups of pig injection samples were prepared:Group A (18 μg 146S antigen),group B (18 μg 146S antigen＋400 μg non-antigenic protein),group C (18 μg 146S antigen＋4 000 μg non-antigenic protein) and group D (blank control,PBS).The cell immune response levels of mice in different vaccine sample groups were compared by lymphocyte proliferation assay and Real-time PCR.The level of humoral immune response produced by the animals (mice and pigs) of each test group was detected and evaluated by a liquid phase blocking ELISA method.The results of mice test showed that the average antibody level of group C was the highest in four test groups at the three test time points after immunization,and the average antibody titers were 4.13,5.83 and 5.50,respectively,while the antibody of group D was the lowest,the average antibody titer average antibody titers were 3.46,5.16 and 4.46 in the antibody detection,respectively;The cell proliferation ability and Th-1 type cytokine IL-6,IFN-β and TNF-α mRNA expression in group C were higher than other groups.The results of pigs test showed that the average antibody level between groups A and B was not significantly different (P>0.05),but the average antibody titers of groups A and B were extremely significantly higher than that of group C (P<0.01).The above results indicated that a small amount of non-antigenic protein had no effect on the immune effect of FMDV-A 146S antigen within a certain range,while a high concentration of non-antigenic protein inhibited the immune effect of FMDV-A 146S antigen.

Key words: non-antigenic proteins; foot-and-mouth disease; 146S antigen; immune effect

CLC Number:

S852.4

LI Ming, ZHANG Yana, GUO Xiaoyu, GAO Xintao, LI Jinxiang, ZHU Hongfei. Effect of Non-antigen Protein of Foot-and-Mouth Disease Vaccine on Immune Effect of 146S Antigen[J]. , 2019, 46(3): 905-912.

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Effect of Non-antigen Protein of Foot-and-Mouth Disease Vaccine on Immune Effect of 146S Antigen

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