口蹄疫、猪瘟、高致病性猪繁殖与呼吸综合征3种疫苗同步两点免疫的田间应用试验

doi:10.16431/j.cnki.1671-7236.2017.08.033

Abstract

Abstract:

In order to explore the optimal immune procedure of foot-and-mouth disease (FMD), classical swine fever (CSF) and highly pathogenic porcine reproductive and respiratory syndrome (HP-PRRS) vaccines in backyard pigs in rural areas, field immune experiment was carried out in two towns. Town A used "332" immune procedure, namely, FMD vaccine and CSF vaccine were simultaneously injected at different sides of the neck, and HP-PRRS vaccine was injected 14 days later. Town B used "321" immune procedure, namely, HP-PRRS vaccine and CSF vaccine were mixed together and injected in one side of the neck, and FMD vaccine was injected in the other side of the neck. FMD, CSF and HP-PRRS antibodies were detected by ELISA, and FMDV, CSFV and HP-PRRSV were detected by RT-PCR. The assessment indexes, including the immune density, immune side-reaction rate, death rate of pigs, positive rate of antibody of backyard pigs, positive rate of antibody of slaughter pigs, positive rate of pathogen of backyard pigs, positive rate of pathogen of slaughter pigs and so on, were collected and analyzed. The results showed that, compared with town A used "332" immune procedure, the HP-PRRS immune density, the positive rate of CSF and HP-PRRS antibody of backyard pigs, the positive rate of HP-PRRS antibody of slaughter pigs were significantly increased (P<0.05), while the death rate and the positive rate of HP-PRRSV of slaughter pigs were significantly decreased (P<0.05) in town B used "321" immune procedure. The other major assessment indexes had no significant difference (P>0.05). The results indicated that the immune efficacy of "321" immune procedure was better than that of "332", and it was worthy to be applied in clinical practice.

Key words: foot-and-mouth disease (FMD); classical swine fever (CSF); highly pathogenic porcine reproductive and respiratory syndrome (HP-PRRS); immune procedure; field experiment; immune efficacy; evaluation

CLC Number:

S858.28

QIN Yong, ZHAO Cong, HUANG Wen-bing, LI Jia-rong, TANG Yun-jiao, BAN Xue-hua, SHI Kai-chuang. Application Experiment in the Field of FMD,CSF and HP-PRRS Vaccines Simultaneously Vaccinated at Two Different Sites[J]. , 2017, 44(8): 2450-2457.

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Application Experiment in the Field of FMD,CSF and HP-PRRS Vaccines Simultaneously Vaccinated at Two Different Sites

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