鹅源副鸡禽杆菌的分离鉴定及致病性研究

doi:10.16431/j.cnki.1671-7236.2023.06.040

Abstract

Abstract: 【Objective】 This study was amied to study biological characteristics of Avibacterium paragallinarum (Apg) in Guangdong province and its differences with Apg strains in China and abroad.【Method】 Suspected cases of infectious rhinitis of geese were collected from Guangdong from December 2019 to January 2022.After specific PCR amplification, bacterial isolation and purification, 16S rRNA gene sequencing and identification, sequence comparison was conducted and phylogenetic tree was constructed to determine the type of the isolated strain, and drug sensitivity test and animal regression test were conducted on the isolated strain.【Result】 A total of 3 strains from goose derived Apg were isolated and identified, named G-AP1, G-AP2 and G-AP3, respectively, which all belonged to NAD-dependent strains.The isolated strain fermented glucose and sucrose to produce acid but not gas, and the oxidase test was positive.The results of phylogenetic tree showed that the serotypes of the three isolates were all type A and were closely related to each other, which were close to most of the isolates in China and distant to the isolates abroad.Isolated strains infected geese, the geese showed facial swelling and runny nose fluid after 24 h and milky white and flocculent nasal fluid flowed out from the swollen part when squeezed after 48 h.The infected geese showed subcutaneous swelling, edema, bleeding, fibrous exudation and caseous exudate accumulated in the sinus cavity.The drug sensitivity test showed that ceftiofurme, ceftianol, amikacin, quinolones, flufenicol and clindamycin had strong growth inhibition on the three isolates in vitro.【Conclusion】 In this study, three strains of Apg from geese were successfully isolated, which could cause infection and infectivity of geese.The results provided a reference for the prevention and control of infectious rhinitis of geese in Guangdong.

Key words: goose; Avibacterium paragallinarum(Apg); isolation and identification; regression test; drug sensitivity test

CLC Number:

S852.61

LU Yukui, LIU Jiaqi, ZHONG Jiacheng, CHEN Jidang, ZHU Wanjun, ZHANG Yishan, ZHANG Jipei. Isolation,Identification and Pathogenicity Analysis of Avibacterium paragallinarum from Geese[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2562-2572.

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Isolation,Identification and Pathogenicity Analysis of Avibacterium paragallinarum from Geese

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