1株鸡源肠炎沙门氏菌的分离鉴定及致病性分析

doi:10.16431/j.cnki.1671-7236.2022.10.042

Abstract

Abstract: 【Objective】 The purpose of the test was to determine the main pathogenic bacteria in the suspected Salmonella disease materials sent by a laying hens farm in Yunnan,and to determine their pathogenicity and drug sensitivity.【Method】 The strains were identified by bacterial isolation and culture,morphological observation,biochemical test,serological identification and molecular methods.The pathogenicity of the isolated bacteria was analyzed through the pathogenicity test of chicken and the detection of virulence genes.The drug resistance of strains was studied by antibiotic sensitivity test.【Result】 The isolated strain formed purple colony on the Salmonella chromogenic medium,and was Gram-negative Brevibacterium and suspected to be Salmonella,named S2.The results of biochemical test showed that the results of lysine decarboxylase,hydrogen sulfide and dulcite were positive,and indol,urease,KCN,sorbitol,salicin,β-galactoside and malonate were negative,and S2 was judged to be a typical Salmonella.The results of serological identification showed that S2 belonged to Salmonella Enteritidis group D,and its antigen formula was 1,9,12:g,m.The results of genetic evolution analysis showed that S2 and Salmonella Enteritidis MT621365 were in the same branch,and the self-development value was 99%.The results of pathogenicity test showed that the isolated strain had strong pathogenicity to chickens,and all chickens died after 6 days of infection (10/10).The results of virulence gene detection showed that S2 carried invJ,hila,ssaB,misL,mgtC,orf319,sopB,spvA,spvB,spvC,spvR,stn and fimA virulence genes,but did not carried sopA and flic virulence genes.The isolated strain S2 was highly sensitive to most antibiotics,and it was sensitive to 12 antibacterial drugs including amoxicillin-clavulanic acid,cefoxitin,tetracycline and so on,but it was resistant to methoxypyrimidine,compound sulfamethoxazole and lincomycin.【Conclusion】 A strain of Salmonella Enteritidis was isolated in this study.It had strong virulence which could cause high mortality of chickens,and it was sensitive to most commonly used antibiotics,but was resistant to methoxypyrimidine,compound sulfamethoxazole and lincomycin.This study provided a certain reference basis for the clinical diagnosis of salmonellosis and the evaluation of drug effectiveness.

Key words: Salmonella Enteritidis; isolation and identification; pathogenicity; drug sensitivity test

CLC Number:

S852.61^＋2

ZHANG Tiantian, LYU Wenliang, LIN Xianhua, LU Yao, XU Haiyan, GU Wei, HAO Muqiang, SHENG Yongjie. Isolation,Identification and Pathogenicity Analysis of a Strain of Salmonella Enteritidis from Chicken[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(10): 4097-4105.

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Isolation,Identification and Pathogenicity Analysis of a Strain of Salmonella Enteritidis from Chicken

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