畜禽粪污中金黄色葡萄球菌和大肠埃希氏菌的LAMP检测及其耐药性分析

doi:10.16431/j.cnki.1671-7236.2020.07.039

Abstract

Abstract: For further understanding of the prevalence and drug resistance of Staphylococcus aureus and Escherichia coli in livestock and poultry manure,the established loop-mediated isothermal amplification technique (LAMP) in livestock and poultry manure was used to detect S.aureus and E.coli in cattle,pig,chicken and duck manure in Shandong province.Then the strains were isolated from the positive samples,and the resistance genes of the multiple resistance strains in the drug sensitivity test were predicted.The results showed that the LAMP method to detect S.aureus and E.coli in livestock and poultry manure was completely consistent with the national standard method,with good repeatability and high specificity.Three strains of S.aureus isolated from 80 samples of livestock and poultry manure were resistant to penicillin.The drug resistance rate of 34 E.coli strains to florfenicol and rifampicin was more than 50%,and the proportion of drug resistance to ampicillin,tetracycline,doxycycline,sulfisoxazole,cephalotin and ceftiofur were more than 25%.The prediction results of drug resistance gene showed that four strains of E.coli in chicken,duck,cow and pig manure carried 10,8,20 and 15 drug resistant genes,respectively.One strain of S.aureus in chicken manure carried 11 drug resistant genes.It indicated that the drug resistance situation of S.aureus and E.coli in livestock and poultry manure of Shandong was severe,the strains were generally multi-drug resistant,and they carried a variety of drug resistance genes.

Key words: livestock and poultry manure; LAMP; Staphylococcus aureus; Escherichia coli; drug sensitivity test; resistance gene

CLC Number:

S852.6

FAN Lixia, YUAN Xuexia, LI Yuanyang, WU Yuanjuan, ZHAO Shancang, ZHANG Bingchun, WANG Wenbo. LAMP Detection and Drug Resistance Analysis to Staphylococcus aureus and Escherichia coli in Livestock and Poultry Manure[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(7): 2325-2335.

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LAMP Detection and Drug Resistance Analysis to Staphylococcus aureus and Escherichia coli in Livestock and Poultry Manure

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