冷季与暖季对牦牛放牧行为及瘤胃微生物多样性的影响

doi:10.16431/j.cnki.1671-7236.2020.07.038

Abstract

Abstract: The aim of the study was to investigate the grazing behavior and rumen microbiology profile of yak in different seasons,in order to provide the scientific basis for improving yak production performance and the animal production transformation efficiency.Nine healthy yaks with similar body weight were selected as the experimental animals,and the trail was conducted in January 2019 and September 2019,respectively.Rumen fluid was collected to analyze the rumen environmental parameters and rumen microbial diversity.The MOOnitor system was adopted to record the grazing behavior.The results showed that the grazing behavior and spatial distribution of yak varies with the season variations of forage yield and nutrition quality.The time spending for rest and rumination (13.378 h/d),grazing (5.174 h/d),and ruminating (8.160 h/d) of yak was significantly decreased in cold season compared with those in warm season (P<0.05),however,the walking time was significantly increased in cold season (P<0.05).The spatial distribution of grazing was more dispersed in cold season.The content of ammonia nitrogen,acetate,propionate,isobutyrate,valerate and total volatile fatty acids in rumen fluid,and the ratio of acetate to propionate were decreased significantly during cold season (P<0.05).However,microbial protein and isovaleric acid content in rumen were significantly increased in cold season (P<0.05).At the level of phylum,Bacteroidetes increased significantly (P<0.05) and Firmicutes decreased significantly (P<0.05) in cold season.At the genus level,cellulose-decomposing bacteria was dominate in rumen fluid of yak in cold season.In conclusion,in cold season,yak reduced the time for grazing,ruminating and rest,and increased the time for walking within a greater space to cope with the lower forage biomass and nutrition quality.In the meantime,the rumen digestion metabolism and microbiology were also changed in cold season in order to improve the energy intake and body utilization efficiency to cope with the deficiency of forage.

Key words: yak; cold season; warm season; grazing behavior; rumen microbiota

CLC Number:

S823.8⁺5

LI Chen, ANUM Ali Ahmad, ZHANG Jianbo, LIANG Zeyi, DING Xuezhi, YAN Ping. Effects of Cold and Warm Season on Grazing Behavior and Rumen Microbial Diversity of Free-range Yak[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(7): 2312-2324.

References

[1] JIA C,WANG H,LI C,et al.Genome-wide detection of copy number variations in polled yak using the Illumina BovineHD BeadChip[J].BMC Genomics,2019,20(1):376.
[2] QIANG Q,GUOJIE Z,TAO M,et al.The yak genome and adaptation to life at high altitude[J].Nature Genetics,2012,44(8):946-949.
[3] 丁路明,龙瑞军,郭旭生,等.放牧生态系统家畜牧食行为研究进展[J].家畜生态学报,2009,30(5):4-9. DING L M,LONG R J,GUO X S,et al.Advances in studies on livestock grazing behaviour in grassland ecosystem[J].Acta Ecologiae Animalis Domastici,2009,30(5):4-9.(in Chinese)
[4] FAHEY G,COLLINS M,et al.Forage quality,evaluation,and utilization[M].Madison:Amer Society of Agronomy,1994.
[5] LIU P,DING L,ZHOU Y,et al.Behavioural characteristics of yaks grazing summer and winter pastures on the Qinghai-Tibetan plateau[J].Applied Animal Behaviour Science,2019,218:104826.
[6] 白彦福,侯尧宸,冯琦胜,等.基于GPS和GIS技术的冬季和夏季牧场牦牛牧食行为研究[J].家畜生态学报,2017,38(2):52-57. BAI Y F,HOU Y C,FENG Q S,et al.Study on yak's grazing behavior in summer and winter pasture using GPS and GIS technology[J].Journal of Domestic Animal Ecology,2017,38(2):52-57.(in Chinese)
[7] 张晓庆,张英俊.绵羊牧食行为对限时放牧加补饲制度的响应[J].畜牧兽医学报,2015,46(11):1994-2001. ZHANG X Q,ZHANG Y J.Response of ingestive behaviour of sheep to restricted time at pasture and indoor feeding[J].Chinese Journal of Animal and Veterinary Sciences,2015,46(11):1994-2001.(in Chinese)
[8] DING L,WANG Y,KREUZER M,et al.Seasonal variations in the fatty acid profile of milk from yaks grazing on the Qinghai-Tibetan plateau[J].Journal of Dairy Research,2013,80(4):410-417.
[9] GIBB M J,HUCKLE C,NUTHALL R,et al.Effect of sward height on intake and grazing behaviour by lactating Holstein Friesian cows[J].Grass & Forage Science,1997,52(3):309-321.
[10] HODGSON J,MILNE J A.The influence of weight of herbage per unit area and per animal upon the grazing behaviour of sheep[J].Proceedings of the European Grassland Federation,1978,431-438.
[11] DING L,LONG R,YANG Y,et al.Behavioural responses by yaks in different physiological states (lactating,dry or replacement heifers),when grazing natural pasture in the spring (dry and germinating) season on the Qinghai-Tibetan plateau[J].Applied Animal Behaviour Science,2007,108(3-4):239-250.
[12] 丁路明,龙瑞军,杨予海,等.秋冬季牧场采用IGER记录器对牦牛24小时采食和反刍行为的记录研究[J].家畜生态学报,2007,3:84-89. DING L M,LONG R J,YANG Y H,et al.Study on grazing and ruminating behaviour of yaks over 24 hours by Iger-recorder in autumn and winter pastures[J].Acta Ecologiae Animalis Domastici,2007,3:84-89.(in Chinese)
[13] AHARONI Y,HENKIN Z,EZRA A,et al.Grazing behavior and energy costs of activity:A comparison between two types of cattle[J].Journal of Animal Science,2009,87(8):2719-2731.
[14] BROSH A,AHARONI Y,SHARGAL E,et al.Energy balance of grazing beef cattle in Mediterranean pasture,the effects of stocking rate and season-2.Energy expenditure as estimated from heart rate and oxygen consumption,and energy balance[J].Livestock Production Science,2004,90(2-3):101-115.
[15] AOAC.Official Methods of Analysis[M].Gaithersburg:Associationof Official Analytical Chemists.2002.
[16] VAN SOEST P V,ROBERTSON J,LEWIS B.Methods for dietary fiber,neutral detergent fiber,and nonstarch polysaccharides in relation to animal nutrition[J].Journal of Dairy Science,1991,74(10):3583-3597.
[17] 张丽英.饲料分析及饲料质量检测技术[M].北京:中国农业大学出版社,2007. ZHANG L Y.Feed Analysis and Feed Quality Testing Technology[M].Beijing:China Agricultural University Press,2007.(in Chinese)
[18] HRISTOV A N,IVAN M,RODE L M,et al.Fermentation characteristics and ruminal ciliate protozoal populations in cattle fed medium-or high-concentrate barley-based diets.[J].Journal of Animal Science,2001,79(2):515-524.
[19] 周建伟.藏羊对青藏高原氮素营养胁迫的适应性研究[D].兰州:兰州大学,2015. ZHOU J W.Adaptability of Tibetan sheep to nitrogen nutrition stress on the Qinghai-Tibet plateau[D].Lanzhou:Lanzhou University,2015.(in Chinese)
[20] KECHIN A,BOYARSKIKH U,KEL A,et al.CutPrimers:A new tool for accurate cutting of primers from reads of targeted next generation sequencing[J].Journal of Computational Biology A Journal of Computational Molecular Cell Biology,2017,24(11):1138-1143.
[21] NEUFELD J D,ENGEL K,CHENG J,et al.Open resource metagenomics:A model for sharing metagenomic libraries[J].Standards in Genomic Sciences,2011,5(2),203-210.
[22] HAAS B J,GEVERS D,EARL A M,et al.Chimeric 16S rRNA sequence formation and detection in Sanger and 454-pyrosequenced PCR amplicons[J].Genome Research,2011,21(3):494-504.
[23] EDGAR R C.UPAR S E:Highly accurate OTU sequences from microbial amplicon reads[J].Nature Methods,2013,10(10):996.
[24] CAPORASO J G,KUCZYNSKI J,STOMBAUGH J,et al.QIIME allows analysis of high-throughput community sequencing data[J].Nature Methods,2010,7(5):335-336.
[25] LOZUPONE C,KNIGHT R.UniFrac:A new phylogenetic method for comparing microbial communities[J].Applied & Environmental Microbiology,2006,71(12):8228-8235.
[26] QUAST C,PRUESSE E,YILMAZ P,et al.The SILVA ribosomal RNA gene database project:Improved data processing and web-based tools[J].Nucleic Acids Research,2013,41:D590-D596.
[27] CHAPMAN D,PARSONS A,COSGROVE G,et al.Impacts of spatial patterns in pasture on animal grazing behavior,intake,and performance[J].Crop Science,2007,47(1):399-415.
[28] KASSILLY F N.Forage quality and camel feeding patterns in Central Baringo,Kenya[J].Livestock Production Science,2002,78(2):175-82.
[29] 米见对.细菌与甲烷菌在牦牛瘤胃中的时间动态及其在消化道的空间分布[D].兰州:兰州大学,2016. Mi J D.Dynamics in rumen and distribution along gastrointestinal tracts of bacteria and methanogen in yak[D].Lanzhou:Lanzhou University,2016.(in Chinese)
[30] 丁路明,龙瑞军,杨予海,等.牦牛夏秋季和冬季各草场牧食行为的研究[J].中国畜牧杂志,2007,5:52-55. DING L M,LONG R J,YANG Y H,et al.Study on grazing behaviour of yaks in summer,autumn and winter[J].Chinese Journal of Animal Science,2007,5:52-55.(in Chinese)
[31] 丁学智,杨超,李晨,等.基于MOOnitor监测系统对青海大通地区无角牦牛冷季放牧行为的研究[J].中国草食动物科学,2018,38(4):44-48. DING X Z,YANG C,LI C,et al.Research on grazing behavior of hornless yaks in cold season in qinghai datong area with monitoring system[J].China Herbivore Science,2018,38(4):44-48.(in Chinese)
[32] 刘培培,丁路明,陈军强.利用GPS跟踪定位系统对祁连山区秋季牧场牦牛和犏牛牧食行为的研究[J].家畜生态学报,2015,36(10):56-60. LIU P P,DING L M,CHEN J Q.Study on the grazing behaviour of yak and cattle-yak in autumn pasture of Qilian mountain by GPS tracking and positioning system[J].Journal of Domestic Animal Ecology,2015,36(10):56-60.(in Chinese)
[33] HOUTERT M F J V.The production and metabolism of volatile fatty acids by ruminants fed roughages:A review[J].Animal Feed Science & Technology,1993,43(3-4):189-225.
[34] SHI F H,FANG L,MENG Q X,et al.Effects of partial or total replacement of maize with alternative feed source on digestibility,growth performance,blood metabolites and economics in limousin crossbred cattle[J].Asian Australasian Journal of Animal Sciences,2014,27(10):1443-1451.
[35] 方雷.饲养方式对夏季牦牛生长性能、屠宰性能、牛肉品质和瘤胃细菌多样性的影响[D].北京:中国农业大学,2015. FANG L.Effects of feeding patterns in summer on yaks' growth performance,carcass traits,meat quality and rumen bacteria diversity[D].Beijing:China Agricultural University,2015.(in Chinese)
[36] 马力,徐世晓,刘宏金,等.不同物候期牧草对放牧牦牛瘤胃内环境参数及瘤胃微生物多样性的影响[J].动物营养学报,2019,31(2):681-691. MA L,XU S X,LIU H J,et al.Effects of forage grass in different phenological periods on ruminal environment parameters and rumen microbial diversity of free-range yak (Bos grunniens)[J].Chinese Journal of Animal Nutrition,2019,31(2):681-691.(in Chinese)
[37] 凌虹,陈红跃,官继承.日粮碳水化合物结构对瘤胃发酵及消化代谢影响的研究进展[J].中国畜牧兽医,2012,39(9):134-137. LING H,CHEN H Y,GUAN J C.Research progress on effects of different proportions of dietary carbohydrates on the ruminal fermentation and metabolism)[J].China Animal Husbandry & Veterinary Medicine,2012,39(9):134-137.(in Chinese)
[38] 牛玉环.高寒植物中不同分子量单宁对牦牛体外瘤胃发酵甲烷产量的影响[D].兰州:兰州大学,2013. NIU Y H.Effect of different molecular weight of tannins from alpine plants on in vitro methane production in yaks[D].Lanzhou:Lanzhou University,2013.(in Chinese).
[39] 丁学智.单宁酸对瘤胃发酵特性及甲烷产量的影响[D].兰州:甘肃农业大学,2006. DING X Z.Effect of tannic acid on in vitro remen fwementation characteristics and methane emission of sheep[D].Lanzhou:Gansu agriclutre University,2006.(in Chinese).
[40] NOLAN J V,LENG R A,DOBOS R C,et al.The production of acetate,propionate and butyrate in the rumen of sheep:Fitting models to 14C-or 13C-labelled tracer data to determine synthesis rates and interconversions[J].Animal Production Science,2014,54(12):2082-2088.
[41] 蔡晶晶,王洪荣,付聪,等.不同NFC/NDF饲粮和硫胺素对奶牛瘤胃代谢的影响[J].动物营养学报,2013,25(9):2012-2020. CAI J J,WANG H R,FU C.et al.Effects of different dietary NFC/NDF and thiamine on rumen metabolism in dairy cows[J].Chinese Journal of Animal Nutrition,2013,25(9):2012-2020.(in Chinese)
[42] 范文斌,李长青,高仙灵,等.放牧蒙古羊瘤胃细菌随季节变化规律的研究[J].中国畜牧兽医,2019,46(8):2315-2321. FAN W B,LI C Q,GAO X L,et al.Study on the Seasonal variation of rumen bacteria in grazing Mongolian sheep)[J].China Animal Husbandry & Veterinary Medicine,2019,46(8):2315-2321.(in Chinese)
[43] 淡瑞芳,张海涛,龙瑞军,等.季节变化对放牧藏系绵羊瘤胃发酵特性及产甲烷菌的影响[J].西北农业学报,2013,22(2):1-6. DAN R F,ZHANG H T,LONG R J,et al.Effects of seasonal shift on quantity of rumen methanogen and fermentation of grazing Tibetan sheep[J].Acta Agriculturae Boreali-occidentalis Sinica,2013,22(2):1-6.(in Chinese)
[44] 魏德泳,朱伟云,毛胜勇.日粮不同NFC/NDF比对山羊瘤胃发酵与瘤胃微生物区系结构的影响[J].中国农业科学,2012,45(7):1392-1398. WEI D Y,ZHU W Y,MAO S Y.Effects of different dietary NFC/NDF ratios on the ruminal fermentation and the changes of the rumen microbial community of goats[J].Scientia Agricultura Sinica,2012,45(7):1392-1398.(in Chinese)
[45] 白萨茹拉,杨红建,李胜利.不同粗饲料组合全混合日粮对泌乳奶牛体外瘤胃挥发性脂肪酸生成动力学特性的影响[J].动物营养学报,2013,25(12):2920-2927. BAI S R L,YANG H J,LI S L.Different roughage combinations in total mixed rations:Effects on kinetic characteristics of in vitro ruminal volatile fatty acid production of lactating dairy cows[J].Chinese Journal of Animal Nutrition,2013,25(12):2920-2927.(in Chinese)
[46] 李旺.瘤胃挥发性脂肪酸的作用及影响因素[J].中国畜牧杂志,2012,48(7):63-66. LI W.The Effect and influence factors of rumen volatile fatty acid[J].Chinese Journal of Animal Science,2012,48(7):63-66.(in Chinese)
[47] 于满满,张美美,孙悠然,等.氨化秸秆与苜蓿组合对其营养成分及瘤胃微生物区系的影响[J].中国饲料,2019,21:19-25. YU M M,ZHANG M M,SUN Y R,et al.Effects of combination of ammoniated straw and trifoliate on nutrients and rumen microflora[J].China Feed,2019,21:19-25.(in Chinese)
[48] 张盼盼,薛树媛,金海,等.不同物候期牧草对放牧绵羊瘤胃发酵指标的影响[J].中国畜牧兽医,2018,45(3):698-704. ZHANG P P,XUE S Y,JIN H,et al.Effects of different phenophase on rumen fermentation of grazing sheep[J].China Animal Husbandry & Veterinary Medicine,2018,45(3):698-704.(in Chinese)
[49] 王炜,崔明九,秦春林,等.基于文献计量的甜高粱研究态势分析[J].草业科学,2016,33(9):1846-1857. WANG W,CUI M J,QIN C L,et al.Status and trends of sweet sorghum research based on bibliometrics[J].Pratacultural Science,2016,33(9):1846-1857.(in Chinese)
[50] GRILLI D J,FLIEGEROVÁ K,KOPEČNÝ J,et al.Analysis of the rumen bacterial diversity of goats during shift from forage to concentrate diet[J].Anaerobe,2016,42:17-26.
[51] 侯明杰,付晓悦,尚占环,等.饲用甜高粱青贮对小尾寒羊瘤胃代谢参数的影响[J].草业科学,2018,35(5):1232-1239. HOU M J,FU X Y,SHANG Z H,et al.Effect of forage sweet sorghum silage on the metabolic parameters in the rumen of Small-tailed Han sheep[J].Pratacultural Science,2018,35(5):1232-1239.(in Chinese)
[52] 吴琼,王思珍,张适,等.基于16S rRNA高通量测序技术分析中国西门塔尔牛瘤胃微生物多样性和功能预测的研究[J].中国畜牧兽医,2019,46(5):1370-1378. WU Q,WANG S Z,ZHANG S,et al.Analysis of rumen microbial diversity and functional prediction of chinese Simmental cattle based on 16S rRNA high-throughput sequencing technology[J].China Animal Husbandry & Veterinary Medicine,2019,46(5):1370-1378.
[53] 魏园,赵晨旭,李心慰,等.不同粗纤维源日粮对梅花鹿瘤胃微生物区系的影响[J].中国兽医学报,2018,38(1):217-221. WEI Y,ZHAO C X,LI X W,et al.Effect of different crude fiber source diets on rumen microflora of sika deer[J].Chinese Journal of Veterinary Science,2018,38(1):217-221.(in Chinese)
[54] 张双奇,王忙生.瘤胃中纤维物质降解规律的研究进展[J].商洛学院学报,2014,28(2):57-61. ZHANG S Q,WANG M S.The research advance of fibrous matter degradation regularity in ruminant[J].Journal of Shangluo University,2014,28(2):57-61.(in Chinese)
[55] MATSUI H,OGATA K,TAJIMA K,et al.Phenotypic characterization of polysaccharidases produced by four Prevotella type strains[J].Current Microbiology,2000,41(1):45-49.
[56] 谢骁.低质粗饲料日粮干预对湖羊瘤胃发酵和微生物菌群的影响[D].杭州:浙江大学,2018. XIE X.Effects of dietary intervention on rumen fermentation and microbiota in sheep fed low quality forage[D].Hangzhou:Zhejiang University,2018.(in Chinese)
[57] HUWS S A,CHIARIOTTI A,SARUBBI F,et al.Effects of feeding Mediterranean buffalo sorghum silage versus maize silage on the rumen microbiota and milk fatty acid content[J].The Journal of General and Applied Microbiology,2012,58(2):107-112.
[58] AYUK J,SHEPPARD M C.Growth hormone and its disorders[J].Postgraduate Medical Journal,2006,82(963):24-30.
[59] PATRA A K,YU Z.Effects of vanillin,Quillaja saponin,and essential oils on in vitro fermentation and protein-degrading microorganisms of the rumen[J].Applied Microbiology and Biotechnology,2014,98(2):897-905.
[60] MITSUMORI M,SUN W.Control of rumen microbial fermentation for mitigating methane emissions from the rumen[J].Asian-Australasian Journal of Animal Sciences,2008,21(1):144-154.

Effects of Cold and Warm Season on Grazing Behavior and Rumen Microbial Diversity of Free-range Yak

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