鹅大肠埃希菌的分离鉴定及系统进化树分析

doi:10.16431/j.cnki.1671-7236.2021.03.026

Abstract

Abstract: In order to provide guidance on the clinical treatment of pathogenic E. coli infection in morbid geese and to prevent and control pathogenic E. coli infection,in this study,spleen,liver and other tissues and organs of diarrhea geese and dead geese were collected aseptically for pathogen isolation and culture,staining observation,biochemical identification,pathogenicity test,partial gene sequencing,phylogenetic tree construction and drug sensitivity test.The results showed that off-white,round,raised,and neatly edged colonies grew on ordinary agar medium,and rose-red,smooth and round colonies grew on MacConkey agar medium.Gram negative bacteria were observed under microscope after Gram staining,the two ends were obtuse and medium in size,most of them were rod-shaped bacteria.The pathogenicity test showed that the isolated strain had high mortality to mice and goslings.The results of the phylogenetic tree showed that the isolated strain had the highest homology with the patient fecal isolate (GenBank accession No.:CP024992.1),reaching 99.5%.In vitro antibacterial test showed that the drug resistance of the strain was serious,and it had strong resistance to most antibiotics.Only ceftiofur had strong antibacterial effect on the isolated strain,and the disease was effectively controlled after rational drug use.This study provided a theoretical basis for the effective control of E.coli in geese,and had important value for the prevention and control of E.coli and other bacterial diseases in large-scale goose farms.

Key words: goose; E. coli; isolation and identification; drug sensitivity test

CLC Number:

S852.61^＋2

ZHANG Bei, JIN Zhenhua, SUN Jinyan, LI Ye, WANG Likun, LU Lingyan, SHI Tongrui. Isolation,Identification and Phylogenetic Tree Analysis of Goose E. coli[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(3): 1020-1026.

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Isolation,Identification and Phylogenetic Tree Analysis of Goose E. coli

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