不同剂量环磷酰胺对昆明小鼠肝肾组织的损伤作用研究

doi:10.16431/j.cnki.1671-7236.2021.02.032

Abstract

Abstract: The aim of this study was to study the injury effect of different doses of cyclophosphamide on liver and kidney of mice.40 healthy female Kunming mice aged 5 weeks were randomly divided into 5 groups,injected intraperitoneally with 40,80,120 and 160 mg/(kg·BW) cyclophosphamide for 3 consecutive days,respectively,except the control group.Liver,kidney and serum were collected on 7th day after injection.The paraffin tissue sections and transmission electron microscope sections were prepared,and the histological changes of liver and kidney were observed.The content or activity of CG,ChE,PA,TBA,ALT,AST,BUN,SCr,UA in serum were detected.The results showed that cyclophosphamide could cause pathological injury to liver and kidney of mice in a dose-dependent manner,and the liver showed injury earlier than the kidney.The observed results of paraffin sections and transmission electron microscopy sections showed that the toxic effects of cyclophosphamide on the liver were mainly manifested in hepatic cord disorders,hepatic sinusoidal dilatation,microbiliary cholestasis,inflammatory cell infiltration and fibrous proliferation in the portal area and around the central vein,hemorrhage between tissues,fatty degeneration and balloon-like degeneration of liver cells,apoptosis and necrotic cells increased.Kidney tissue damages were mainly manifested in hemorrhage and fibrosis,renal tubular epithelial cell cytoplasmic vacuolation,mitochondrial damage,nuclear pyknosis,and thickening of the basement membrane of epithelial cells.Cyclophosphamide did not cause serious damage to the bladder.Compared with the control group,serum ChE activites and PA contents in 40-160 mg/(kg·BW) groups,AST activities in 120-160 mg/(kg·BW) groups,BUN contents in 80-160 mg/(kg·BW) groups,and UA level in 80 mg/(kg·BW) group were increased significantly (P<0.05).The other biochemical indicators were not significantly different from the control group (P>0.05).The results showed that cyclophosphamide injection at a dose of 80-120 mg/(kg·BW) could cause obvious pathological damage to liver and kidney tissues and cells in Kunming mice.The results of the study could provide an experimental basis for the selection of appropriate cyclophosphamide in establishment of animal liver immunosuppression model.

Key words: cyclophosphamide; mice; liver and kidney function; pathological changes

CLC Number:

S852.3

WANG Yifei, CAO Nan, XU Danning, ZHENG Xunxun, WU Xiaotong, LIANG Shuqi, HUANG Yunmao, TIAN Yunbo, LI Wanyan. Study on the Injury Effect of Different Doses of Cyclophosphamide on the Liver and Kidney Tissues of Kunming Mice[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(2): 695-703.

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Study on the Injury Effect of Different Doses of Cyclophosphamide on the Liver and Kidney Tissues of Kunming Mice

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