RF酰胺相关肽3对大鼠脾脏形态结构及免疫功能的影响

doi:10.16431/j.cnki.1671-7236.2020.04.029

Abstract

Abstract: The purpose of this study was to investigate the effects of different RFRP-3 treatment on morphological structure and immune function of spleen in SD rats.The experiment was comprised of two parts:10 SD rats were chosen for the study about the distribution of RFRP-3 and GPR147.In the other part,30 SD rats were randomly assigned into 3 groups.They were control group (0.9% NaCl),low-dose group and high-dose group (1 and 10 μg/100 μL,total 200 μL of RFRP-3).Intraperitoneal injection was continued for 14 days.The expression of RFRP-3 and its receptor (GPR147) were investigated by using semi-quantitative RT-PCR and Western blotting.The distribution of RFRP-3 and GPR147 were determined by using immunohistochemistry.Blood was collected and serum was isolated to detect immune-related factors by using a protein chip array after anesthesia.Spleen length was measured and spleen index was calculated after the autopsy.The effect of RFRP-3 on morphology of spleen was detected by HE staining.The expression of IL-β and TNF-α mRNA in spleen was detected by Real-time quantitative PCR.Results showed that no RFRP-3 mRNA expression was observed in spleen,GPR147 mRNA was moderately expressed.The results of Western blotting showed that both RFRP-3 and GPR147 were detected in spleen.RFRP-3 was mainly distributed in red pulp,while GPR147 was mainly distributed in white pulp of spleen.The concentrations of IL-1α,IL-1β,MCP-1 and TNF-α were significantly increased in the rats treated with RFRP-3.The length of spleen increased and spleen index increased,too.The results of HE showed that the white pulp area expanded with more splenic sinus and the red blood cell in red pulp were evidently increased.The results of Real-time quantitative PCR showed that the expression of IL-1β and TNF-α mRNA in spleen of SD rats were extremely significantly or significantly increased (P<0.01;P<0.05).The above results showed that RFRP-3 might act on the spleen via GPR147 through the paracrine manner and promote immune response.

Key words: RFRP-3; rat; spleen; immune function; cytokines

CLC Number:

S852.1

ZHANG Duoni, HUO Konglin, LI Changle, ZHANG Xin, SONG Xingxing, HU Wen, LUO Rongrong, XU Wenhao, CHEN Lei, CHEN Xinyi, LI Xun, WANG Xiaoye. Effects of RFRP-3 on Morphological Structure and Immune Function of Spleen in Rats[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(4): 1225-1232.

References

[1] TSUTSUI K,SAIGOH E,UKENA K,et al.A novel avian hypothalamic peptide inhibiting gonadotropin release[J].Biochemical and Biophysical Research Communications,2000,275(2):661-667.
[2] GEORGE J T,HENDRIKSE M,VELDHUIS J D,et al.Effect of gonadotropin-inhibitory hormone on luteinizing hormone secretion in humans[J].Clinical Endocrinology,2017,86(5):731-738.
[3] TSUTSUI K,UBUKA T,BENTLEY G E,et al.Gonadotropin-inhibitory hormone (GnIH):Discovery,progress and prospect[J].General and Comparative Endocrinology,2012,177(3):305-314.
[4] MUÑOZ-CUETO J A,PAULLADA-SALMERÓN J A,ALIAGA-GUERRERO M,et al.A journey through the gonadotropin-inhibitory hormone system of fish[J].Frontiers in Endocrinology,2017,8:285.
[5] UBUKA T,PARHAR I.Dual actions of mammalian and piscine gonadotropin-inhibitory hormones,RFamide-related peptides and LPXRFamide peptides,in the hypothalamic-pituitary-gonadal axis[J].Frontiers in Endocrinology,2018,8:377.
[6] UBUKA T,PARHAR I S,TSUTSUI K.Gonadotropin-inhibitory hormone mediates behavioral stress responses[J].General and Comparative Endocrinology,2018,265:202-206.
[7] 汪瑶,李珣,李敏婕,等.GnIH对母猪生殖调控的研究[J].中国农业科学,2014,47(18):3716-3724. WANG Y,LI X,LI M J,et al.Regulation of GnIH on reproduction of female pig[J].Scientia Agricultura Sinica,2014,47(18):3716-3724.(in Chinese)
[8] WANG X,LI X,HU C.Distribution of gonadotropin-inhibitory hormone (GnIH) in male Luchuan piglets[J].Gene Expression Patterns,2018,28:42-53.
[9] BRONTE V,PITTET M J.The spleen in local and systemic regulation of immunity[J].Immunity,2013,39(5):806-818.
[10] ANJUM S,KRISHNA A,TSUTSUI K.Possible role of GnIH as a mediator between adiposity and impaired testicular function[J].Frontiers in Endocrinology,2016,7:6.
[11] 戴小宇,朱海,张晓冬,等.纤维蛋白封闭剂对SD大鼠连续腹腔注射给药14天的毒性研究[J].药学实践杂志,2015,33(3):231-234. DAI X Y,ZHU H,ZHANG X D,et al.Repeat-dose toxicity study of fibrin sealant in SD rats[J].Journal of Pharmaceutical Practice 2015,33(3):231-234.(in Chinese)
[12] 刘晶.兔GnIH及其受体基因的克隆和mRNA在组织器官的表达[D].南京:南京农业大学,2011. LIU J.The cloning and mRNA expression of GnIH and its receptors in rabbit[D].Nanjing:Nanjing Agricultural University,2011.(in Chinese)
[13] 霍孔林,李昀,王晓晔,等.GnIH在陆川仔公猪泌尿生殖系统及内分泌免疫系统中的分布[J].西北农林科技大学学报(自然科学版),2016,44(12):16-20. HUO K L,LI Y,WANG X Y,et al.Distribution of gonadotropin-inhibitory hormone (GnIH) in urinary,reproductive,endocrine and immune[J].Journal of Northwest A&F University (Natural Science Edition),2016,44(12):16-20.(in Chinese)
[14] IWASA T,MATSUZAKI T,MURAKAMI M,et al.Developmental changes in the mammalian gonadotropin-inhibitory hormone (GnIH) ortholog RFamide-related peptide (RFRP) and its cognate receptor GPR147 in the rat hypothalamus[J].International Journal of Developmental Neuroscience,2012,30(1):31-37.
[15] 李珣.母猪GnIH及受体GPR147基因克隆、mRNAs在全身组织及发情周期中在生殖轴表达模式的研究[A].中国畜牧兽医学会动物解剖学及组织胚胎学分会第十七次学术研讨会论文集(下)[C].2012. LI X.Gonadotropin-inhibitory hormone (GnIH) and its receptor in the female pig:cDNA cloning,expression in tissues and expression pattern in the reproductive axis during the estrous cycle[A].Proceedings of the 17th Symposium of the branch of animal anatomy,histology and embryology,Chinese society of animal husbandry and veterinary medicine[C].2012.(in Chinese)
[16] TANRIVERDI F,SILVEIRA L F,MACCOLL G S,et al.The hypothalamic-pituitary-gonadal axis:Immune function and autoimmunity[J].The Journal of Endocrinology,2003,176(3):293-304.
[17] FERRERO-MILIANI L,NIELSEN O H,ANDERSEN P S,et al.Chronic inflammation:Importance of NOD2 and NALP3 in interleukin-1β generation[J].Clinical&Experimental Immunology,2007,147(2):227-235.
[18] KUSUMI S,KOGA D,KANDA T,et al.Three-dimensional reconstruction of serial sections for analysis of the microvasculature of the white pulp and the marginal zone in the human spleen[J].Biomedical Research,2015,36(3):195-203.
[19] VARGA I,BABALA J,KACHLIK D.Anatomic variations of the spleen:Current state of terminology,classification,and embryological background[J].Surgical and Radiologic Anatomy,2018,40(1):21-29.
[20] 何嘉,郭建辉,梁栋,等.健脾益肺口服液对慢阻肺"肺脾气虚证"大鼠脾脏指数和胸腺指数的影响[J].海南医学,2014,25(10):1412-1414. HE J,GUO J H,LIANG D,et al.Influence of spleen-strengthening and lung-tonifying liquid on spleen index and thymus index of COPD in rats with lung and spleen qi deficiency syndrome[J].Hainan Medical Journal,2014,25(10):1412-1414.(in Chinese)
[21] ATHANASSAKIS I,ICONOMIDOU B.Cytokine production in the serum and spleen of mice from day 6 to 14 of gestation:Cytokines/placenta/spleen/serum[J].Journal of Immunology Research,1995,4(4):247-255.

Effects of RFRP-3 on Morphological Structure and Immune Function of Spleen in Rats

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