紫草素抑制炎症促进驴皮伤口愈合的效果

doi:10.16431/j.cnki.1671-7236.2020.07.036

Abstract

Abstract: The experiment investigated the effect of alkannin gel on wound healing of donkey in order to provide reference for the treatment of skin scars.In vitro cultured donkey macrophages were treated with different concentrations of alkannin,the cells mRNA and supernatant protein expression levels of TGF-β1,IL-10,TNF-α and IL-6 were detected respectively to determine the optimal concentration of alkannin.The skin wound model of donkey was established,and the skin wound was coated with seaweed gel.The wound healing was observed by taking photos and HE staining,and the expression levels of wound healing related genes and serum in the skin of donkey were detected.The results showed that compared with the control group,the addition of alkannin at different concentrations could reduce the expression of related inflammatory factors,among which the 10 μmol/L alkannin group had the best effect.On the 11th day,the wound shrinkage rate of the alkannin group was extremely significant higher than the control group (P<0.01).HE staining showed that the tissues in alkannin were gradually arranged on day 11. At 60th day,the thickness of the superficial cortex in the alkannin group decreased relatively. Real-time quantitative PCR showed that at the 11th day,the expression levels of TGF-β1 and IL-10 mRNA in the alkannin group were extremely significantly increased (P<0.01),while the expression levels of TNF-α and IL-6 mRNA were extremely significantly decreased (P<0.01).At the 3rd day,the serum concentrations of TGF-β1 and IL-10 in the alkannin group were increased,and the concentrations of TNF-α and IL-6 were extremely significantly decreased (P<0.01).At day 7,the concentration of TGF-β1 in the alkannin group was significantly decreased (P<0.05),the concentration of TNF-α was extremely significantly decreased (P<0.01),and the concentration of IL-10 was significantly increased (P<0.05).Therefore,the treatment of donkey skin wound healing with 10 μmol/L alkannin gel had certain anti-inflammatory effect,which promoted the situation of wound healing.

Key words: alkannin; wound healing; scar; inflammatory cytokines

CLC Number:

S822.22

LIANG Gong, WANG Yanping, MIAO Kai, WANG Lu, WANG Xiaolu, ZENG Weibin. Effect of Alkannin on Accelerating Healing Wounds of Donkey Skin by Suppressing Inflammation[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(7): 2295-2303.

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Effect of Alkannin on Accelerating Healing Wounds of Donkey Skin by Suppressing Inflammation

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