鸡蛔虫凉山州分离株ITS和5.8SrDNA序列测定及种系发育分析

doi:10.16431/j.cnki.1671-7236.2015.12.009

Abstract

Abstract: The internal transcribed spacer (ITS) and 5.8S ribosomal DNA (5.8S rDNA) of roudworm (Ascaridia galli, A.galli) samples collected from Liangshan,Sichuan were amplified by PCR using a pair of conserved primers (BD1 and BD2),then sequenced and analyzed the sequences,phylogenetic trees based on ITS-1 and ITS-2 were reconstructed using Mega 5.0 software.The results showed that ITS and 5.8S rDNA sequences of Ascaridia galli were 974 to 989 bp,with homology among these sequences was 98.9% to 100.0%.The sequences of ITS-1,5.8S rDNA and ITS-2 were 473 to 481,157 and 337 to 359 bp,respectively.With homologies of 98.5% to 100.0%,100.0% and 98.5% to 100.0%,respectively.The phylogenetic trees showed that all the Ascaridia galli isolates were formed the same cluster,and could identify with other Ascaridida.The results indicated that there was no significant variation in ITS-1 and ITS-2 sequences within Ascaridia galli,while inter-species difference was obvious,so they could be used as genetic marker to identify the species of Ascaridia galli,and provided the foundation for further research on molecular classification,epidemiological survey and population genetics of Ascaridia galli.

Key words: Ascaridia galli; ITS; 5.8S rDNA; phylogenetic tree

CLC Number:

HAO Gui-ying, HE Xue-qian. Sequencing and Phylogenetic Analysis of ITS and 5.8S rDNA of Ascaridia galli Isolated from Liangshan[J]. , 2015, 42(12): 3167-3172.

References

[1] Abdelqader A,Gauly M,Wollny B A,et al.Prevalence and burden of gastrointestinal helminthes among local chickens,in Northern Jordan[J]. Prev Vet Med,2008,85(1-2):17-22.
[2] 牛庆丽,罗建勋,殷宏.转录间隔区(ITS)在寄生虫分子生物学分类中的应用及其进展[J].中国兽医寄生虫病,2008,16(4):41-47.
[3] 李金平,张浩吉,梁详解,等.鸽蛔虫ITS rDNA的克隆与序列分析[J].湖北农业科学,2012,51(18):4138-4140.
[4] 林瑞庆,蔡天城,吴桂英,等.鸡蛔虫ITS rDNA的PCR扩增克隆及序列分析[J].中国兽医杂志,2008,44(3):24-25.
[5] 胡鹏辉.斑马蛔虫ITS及5.8S rDNA的克隆及进化分析[J].中国动物检疫,2013,30(12):62-65.
[6] 陈绚姣,唐志玲,陈武,等.大熊猫蛔虫ITS及5.8S rDNA序列的克隆与分析[J].野生动物,2011,32(6):332-335.
[7] 王培园,陈武,贺现辉,等.华南虎蛔虫ITS及5.8S rDNA序列扩增及分析[J].畜牧与兽医,2010,42(9):74-76.
[8] 周荣琼,夏庆友,黄汉成,等.犬弓首蛔虫ITS1基因的克隆及序列分析[J].中国预防兽医学报,2010,32(10):818-820.
[9] 李广生,张莹,盛中华,等.狮体内蛔虫的分子生物学鉴定[J].黑龙江八一农垦大学学报,2012,24(6):26-28.
[10] 吴晓斌,张志宏,谢东.小耳花猪蛔虫ITS及5.8S rDNA序列扩增与分析[J].广东畜牧兽医科技,2012,37(5):37-39.
[11] 王培园,陈武,贺现辉,等.棕蓑猫蛔虫ITS及5.8S rDNA序列的克隆与分析[J].中国兽医科学,2010,40(10):1013-1016.
[12] 萨姆布鲁克 J,拉塞尔 D W.黄培堂,王嘉玺,朱厚础,等译.分子克隆实验指南[M].第3版.北京:科学出版社,2002.
[13] Jacobs D E,Zhu X Q,Gasser R B,et al.PCR-based methods for identification of potentially zoonotic ascaridoid parasites of the dog,fox and cat[J].Acta Tropica,1997,68(2):191-200.
[14] Zhu X Q,Jacobs D E,Chilton N B,et al.Molecular characterization of a Toxocara variant from cats in Kuala Lumpur,Malaysia[J].Parasitology,1998,117(2):155-164.
[15] Zhu X Q,D'Amelio S,Palm H W,et al.SSCP-based identification of members within the Pseudoterranova decipiens complex (Nematoda:Ascaridoidea:Anisakidae) using gentic markers in the internal transcribed spacers of ribosomal DNA[J].Parasitology,2002,124(6):615-623.
[16] 何光志,牛李丽,杨光友,等.大熊猫等八种野生珍稀动物蛔虫ITS-2基因的序列分析[J].中国兽医科学,2008,38(11):933-938.
[17] 彭仕明,黄勉,陈武.棕熊蛔虫ITS rDNA的PCR扩增与序列分析[J].中国兽医寄生虫病,2008,16(3):1-5.
[18] 贺现辉,陈武,何乐天,等.美洲狮和亚洲黑熊蛔虫ITS序列扩增及序列分析[J].中国畜牧兽医,2010,37(10):41-45.
[19] Van H L,Blair D,Agatsuma T.Intra and inter-specific variation in nuclear ribosomal internal transcribed spacer 1 of the Schistosoma japonicum species complex[J]. Parsitology,1998,116(4):311-317.
[20] 林瑞庆,吕晓生,邓艳,等.犬复孔绦虫ITS及5.8S rDNA的PCR扩增、克隆及序列分析[J].热带医学杂志,2007,7(2):109-111.

Sequencing and Phylogenetic Analysis of ITS and 5.8S rDNA of Ascaridia galli Isolated from Liangshan

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	ZHANG Rui, MU Chunyu, TANG Qingping, BU Zhu, YANG Mingjun, XIA Aiping, YANG Xiaoming. Polymorphism of GnIH Gene and Its Correlation with Egg-laying Traits in Pigeons [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1412-1421.
[2]	REN Jiangang, GUAN Peng, HUANG Jianzhi, SHENG Yueyun, BAO Tiezhu, MA Zhiping, QIAN Wenxi. Effects of Dietary Energy Level on Growth-fattening Performance and Serum Biochemical Indices of Angus Bulls [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 966-976.
[3]	LONG Anju, YANG Suan, AI Jinxin, WENG Jimei, ZHANG Yiyu, CAI Huifen, LUO Weixing. Polymorphism of MC4R Gene and Its Association with Growth Traits in Qianbei Ma Goats [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 198-209.
[4]	YANG Man, LIU Hai, ZHANG Run, HU Ziping, NIU Naiqi, WANG Lixian, ZHANG Longchao. Association Analysis of Polymorphism of MYH3 and MYH13 Genes with Meat Quality Traits in Beijing Black Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3428-3437.
[5]	ZHAO Fengmiao, GUO Ting, ZHOU Yaping, ZHAO Hongmei, WANG Yuchen, TIAN Guangyuan, SUN Yajie, BIAN Yuchen, YU Jialiang, HAO Yongqing. Pathogenicity Analysis of Bovine Viral Diarrhea Virus and Immune Effect Evaluation of E2 Protein in New Zealand White Rabbits [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3549-3558.
[6]	YANG Tao, ZHANG Mingjie, XU Ran, ZHANG Han, ZHANG Mengmeng, WU Keliang. Study on Genetic Evaluation of Reproductive and Growth Traits in Pigs by BLUP Method [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 2971-2981.
[7]	TANG Bihui, PAN Lu, LI Haiying, ZHAO Xiaoyu, WU Yingping, JIANG Teng, DING Yawen, WU Linan, CAO Yan, MEI Zhiyong. Genome-wide Selection Signal Analysis of Laying Traits in Geese [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 3015-3025.
[8]	BAI Shaocheng, ZHOU Juan, JIN Rongshuai, WANG Fan, LU Tingting, TANG Xianwei, ZHAO Bohao, WU Xinsheng, CHEN Yang. Cloning and Bioinformatics Analysis of CYP11A1 Gene in New Zealand White Rabbits and Its Effects on Reproduction-related Genes [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2484-2496.
[9]	TIE Yanan, YANG Xixi, WANG Zhiliang, WANG Xinrong. Morphological and Histological Characteristics of Testis and Epididymis of Hyla Rabbits at Different Ages Before Sexual Maturity [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2645-2653.
[10]	XUE Qian, LI Guohui, XING Weijie, ZHOU Chenghao, ZHANG Huiyong, YIN Jianmei, ZHU Yunfen, CAO Yuxia, SU Yijun, HAN Wei. Screening of lncRNA Related to Inbreeding Depression of Reproduction in Chickens [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2176-2185.
[11]	MA Yanru, YU Yongsheng, CAO Yang, QIN Lihong, ZHAO Yumin, WU Jian. Polymorphism of PDK4 Gene and Its Association with Meat Quality Traits in Chinese Steppe Red Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2186-2194.
[12]	CHAI Zhixin, WU Zhijuan, WANG Jikun, ZHANG Chengfu, ZHONG Jincheng, XIN Jinwei. Polymorphisms of CTGF and Gprin3 Genes and Their Association with Body Size Traits in Yaks [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2216-2227.
[13]	WANG Zhiliang, YANG Xixi, TIE Yanan, FU Xiaoyu, WANG Xinrong. Effects of Superovulation on Morphology and Hormone Levels of Reproductive Organs in Rabbits [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2250-2258.
[14]	SHAO Yandi, JIANG Qiufei, FENG Yuan, WANG Yu, ZHANG Juan, ZHOU Zihang, WANG Weizhen, YU Baojun, FENG Xiaofang, CHEN Yafei, GU Yaling. Research Progress on Candidate Genes of Cattle Reproductive Traits [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2270-2278.
[15]	FAN Qiuli, RUAN Dong, JIANG Shouqun, CHENG Zhonggang, WANG Hanhua. Study on Dietary Crude Protein Requirement of Silky Fowl During 26 to 45 Days of Age [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(5): 1727-1737.