超排处理对家兔生殖器官形态及激素水平的影响

doi:10.16431/j.cnki.1671-7236.2022.06.025

Abstract

Abstract: 【Objective】 The aim of this expremient was to study the effect of superovulation on the changes of the morphology and hormone levels of the reproductive organs of rabbits.【Method】 Twelve rabbits were divided into experimental group and control group, 6 rabbits in each group.The rabbits in the experimental group was injected 70 IU pregnancy mare serum gonadotropin(PMSG) into the inner thigh, 72 h after the injection, 100 IU human chorionic gonadotropin (hCG) was injected at the same injection point.The control group was injected with the same volume normal saline at the same time.Rabbits in both the experimental group and the control group were caged with male rabbits to stimulate ovulation.After 24 hours, the rabbits were killed and the ovaries, fallopian tubes and uterus were collected, the length, width and thickness of ovary, the length, width and circumference of uterus, the thickness of uterine wall, the length of fallopian tube, the width of funnel, isthmus and ampulla were measured.The ovaries were made into tissue sections, and the changes of ovarian follicles were studied by HE staining.The ear vein blood of rabbits in the control group and the experimental group were collected before PMSG injection, hCG injection and death, inhibin (INH), follicle-stimulating hormone (FSH), luteinizing hormone (LH), estradiol (E₂) and progesterone (P₄) in serum were detected by ELISA.【Result】 Compared with control group, the weight, length, width, thickness, the number of hyperemic follicles, the number of ovulation points and the number of atretic follicles of ovarian tissues in the experimental group were extremely significantly increased (P<0.01);The diameter of secondary follicles in the ovary was significantly decreased (P<0.05);The length of the fallopian tubes, the width of the ampulla of the fallopian tubes and the length of the uterus were significantly increased (P<0.05);The width of the infundibulum of the fallopian tubes and the length of uterus were significantly increased (P<0.01).At three different blood collection times, the serum INH level of the rabbits in control group showed an increasing trend, Before hCG injection and before death, the INH content in the experimental group was significantly lower than that in control group (P<0.05).Before hCG injection, the serum FSH content of the experimental group was significantly higher than that of control group (P<0.05), while the content of FSH in serum of the experimental group was significantly lower than that of the control group before death(P<0.05).Before hCG injection and before death, the content of LH in serum of the experimental group was significantly higher than that of control group (P<0.05).There was no significant difference in the contents of P₄ and E₂ between the experimental group and the control group at each time point (P>0.05).【Conclusion】 The superovulation treatment of 70 IU PMSG+100 IU hCG increased the ovarian volume and the number of ovulation points on the surface of rabbits, and cooperated with endogenous hormones to act on the ovary to obtain a better superovulation effect in rabbits.

Key words: rabbits; superovulation; reproductive organs; histology; reproductive hormones

CLC Number:

S829.1

WANG Zhiliang, YANG Xixi, TIE Yanan, FU Xiaoyu, WANG Xinrong. Effects of Superovulation on Morphology and Hormone Levels of Reproductive Organs in Rabbits[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2250-2258.

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Effects of Superovulation on Morphology and Hormone Levels of Reproductive Organs in Rabbits

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