甘肃省部分地区兔艾美耳球虫分子流行病学调查研究

doi:10.16431/j.cnki.1671-7236.2021.05.025

Abstract

Abstract: In order to investigate the prevalence,species of Eimeria and risk factors in rabbits in Gansu province,in this study,592 fecal samples of 5 different species of rabbits were collected from Pingliang,Jiuquan and Wuwei cities of Gansu province,and the genomic DNA of each sample was extracted.The Eimeria rabbit species-specific PCR method was used to amplify the Eimeria rabbit gene fragment based on the ribosomal first inner transcribed spacer (ITS1) primers.The results showed that overall prevalence of Eimeria in rabbits was 50%(296/592).Sequencing analysis showed that a total of 7 different Eimeria species in rabbits were identified,including E.magna (24.5%,145/592),E.intestinalis (4.1%,24/592),E.media (11.5%,68/592),E.perforans (19.8%,117/ 592),E.vejdovskyi (24.5%,145/592),E.exigua (4.9%,29/592) and E.coecicola (0.84%,5/592).The E.magna,E.vejdovskyi and E.perforans were the dominant species in rabbits.Moreover,multiple infections had been found,and up to quadruple infections.Statistical analysis showed that region (P<0.05),breed (P<0.05) and age (P<0.05) were the risk factors affecting the prevalence of Eimeria in rabbits,suggesting that we should take effective measures to prevent and control these three factors.The present study revealed the prevalence,Eimeria species and risk factor in rabbits infection in some areas of Gansu province.The results provided scientific basis information for the prevention and control of Eimeria in rabbits in Gansu province.

Key words: Eimeria spp.; rabbits; infected species; risk factors

CLC Number:

S855.9

CHEN Hong, LI Taoshan, ZOU Yang, LIANG Xiaxia, CAO Fuqiong, ZHU Xingquan, SUN Xiaolin. Molecular Epidemiology Investigation of Eimeria spp.in Rabbits in Some Areas of Gansu Province[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(5): 1735-1744.

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Molecular Epidemiology Investigation of Eimeria spp.in Rabbits in Some Areas of Gansu Province

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