抑制素α主动和被动免疫对哈萨克羊生殖激素含量的影响

doi:10.16431/j.cnki.1671-7236.2021.02.028

Abstract

Abstract: In order to study the effect of inhibin α (INHα) on reproductive hormone content of Kazakh sheep by active and passive immunity,the INHα protein was expressed and purified from INHα recombinant plasmid strain and then injected into Xinjiang camel to prepare camel anti-INHα polyclonal antibody.The antibody titer was detected and the specificity of the antibody was verified.45 adult Kazakh sheep (3 to 5 years old),with similar estrus and interestrus,were randomly divided into 3 groups,which were respectively used as the INHα polyclonal antibody immunization group (group A),the INHα recombinant protein immunization group (group B) and the control group (group C).These sheep were immuned three times every ten days.The ELISA method was used to detect five reproductive hormones that had important functions in sheep reproduction:follicle stimulating hormone (FSH),luteinizing hormone (LH),pregnancy ketone (P₄),estrogen (E₂),INH (inhibin).Blood biochemical indicators were detected.The results showed that the optimal induction concentration of IPTG was 0.6 mmol/L,and a higher yield of INHα inclusion body protein was induced at 4 h.The INHα protein was highly purified and had good immunogenicity.The prepared INHα antibody had a titer of 1:512 000,and the antibody could specifically bind to INHα protein.It showed that an immunogenic INHα protein was successfully prepared and after further verification.The camel anti-INHα polyclonal antibody with high antibody titer was successfully prepared.After immunization,the LH,P₄ content of group A and FSH,LH,P₄,E₂,INH content of group B compared with group C were not significantly different (P>0.05),while the FSH and E₂ contents of group A were significantly higher than those of group C (P<0.05),the INH content was significantly lower than that of group C (P<0.05).Through the detection of blood biochemical indicators,it was found that the experimental animals had no adverse symptoms after the immunization of the two immune preparations of INHα protein and INHα antiserum.The results showed that both INHα antigens could have a good effect on the secretion of blood reproductive hormones in Kazakh sheep,and the INHα antiserum had a better immune effect.

Key words: INHα; immune; Kazakh sheep; polyclonal antibody; reproductive hormones

CLC Number:

S813.3

Lina·Shaken, Baheti·Boday, YANG Hua, XIE Jingang, DONG Qiaoyan, Wurelihazi·Hazihan. Effects of Inhibin α on Reproductive Hormone Content in Kazakh Sheep by Active and Passive Immunity[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(2): 658-667.

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Effects of Inhibin α on Reproductive Hormone Content in Kazakh Sheep by Active and Passive Immunity

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