›› 2019, Vol. 46 ›› Issue (1): 279-286.doi: 10.16431/j.cnki.1671-7236.2019.01.033

• Preventive Veterinary Medicine • Previous Articles     Next Articles

Preliminary Study on Immune Effect of Rabies Virus Vaccine Strain with Gene Rearrangement

CHEN Kaiyun, WEN Zhaohai, ZHAI Shaohua, ZHOU Haiying, HU Yuan, JIAN Zijian   

  1. College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi 830052, China
  • Received:2018-05-14 Online:2019-01-20 Published:2019-01-19

Abstract:

To explore whether gene rearrangement rabies virus (RV) vaccine could stimulate the production of RV antibody IgG in mice,and compare the IgG level and safety performance in different immune modes and different immune doses of attenuated RV vaccine,28 35-day-old Kunming mice with (20±3)g were chosen and assigned to 7 groups:Group 1 (low dose oral group,50 μL compound adjuvant +50 μL RV),group 2 (middle dose oral group,150 μL compound adjuvant +150 μL RV),group 3 (high dose oral group,300 μL compound adjuvant +300 μL RV),group 4 (low dose injection group,50 μL compound adjuvant +50 μL RV),group 5 (middle dose injection group,150 μL compound adjuvant +150 μL RV),group 6 (high dose injection group,300 μL compound adjuvant +300 μL RV) and group 7 (oral control group,300 μL compound adjuvant +300 μL SRV9 strain).Immunisation was performed at 0,7 and 14 d,respectively,and the IgG level of mice in each immune group was detected by ELISA kit,and the liver,kidney,lung,brain tissues of the immune groups were collected to evaluate the safety of the vaccine at the end of immunization test.The results showed that IgG was produced in each immune group.The rate of IgG produced by intramuscular injection was higher than that of oral immunization,but intramuscular vaccination could lead to death of mice.The IgG level produced by 600 μL oral immunization group was significantly higher than 100 and 300 μL oral immune groups and parental strains SRV9 control group (P < 0.05),which indicated that increasing oral immunization dose would increase the level of antibody produced by oral immune and would not cause adverse reactions.Histopathological examination found that the liver,kidney,lung and brain tissues of each oral immune group were normal and did not see pathological changes,indicating that the gene rearrangement RV had less side effects and had better safety.It provided a new direction for the development of a new type of RV attenuated oral vaccine.

Key words: gene rearrangement rabies virus vaccine strain; immunization; antibody; pathological histology observation

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