抗菌肽BSN-37的抑菌活性及其稳定性分析

doi:10.16431/j.cnki.1671-7236.2019.01.034

Abstract

Abstract:

The aim of the experiment was to study the bacteriostatic activity and stability of the antimicrobial peptide BSN-37.The minimum inhibitory concentration (MIC) of BSN-37 against several bacteria were detected with serial dilution method.The bactericidal dynamics of BSN-37 against Escherichia coli (E.coli) CVCC1568 were measured by colony counting method.The salt ion stability,acid-alkali stability,serum stability,pancreatic enzyme stability,thermal stability,repeated freeze-thaw stability,maintain stability at room temperature,and efficacy stabilization stability were analyzed through diffusion method.The results showed that the MIC of the antimicrobial peptide BSN-37 against Gram negative bacteria (E.coli and Salmonella) was 1.5625 to 25 μg/mL,and the MIC of BSN-37 against Gram positive bacteria (Bacillus subtilis and Bacillus pumilus) were 1.5625 to 12.5 μg/mL.But BSN-37 had no bacteriostatic activity against fungi (Candida albicans).The antimicrobial peptide BSN-37 could completely kill E.coli CVCC1568 within 90 min.In addition to the Fe²⁺ and pancreatic enzymes could affect the bacteriostatic activity of antimicrobial peptide BSN-37,at other conditions including the high temperature (75 to 100℃),150 to 250 mmol/L high concentration of salt ions (Na⁺,K⁺,Ca²⁺ and Mg²⁺) and 20% to 25% of saturation concentration of Cu²⁺,pH 4 to 10,20% to 25% of serum,10 to 12 times of repeated freezing and thawing,preservation for 10 to 12 days at room temperature,BSN-37 could still maintain good bacteriostatic activity.Efficacy maintain time of BSN-37 was more than 7.5 days.The results provided a theoretical basis for the clinical application of antimicrobial peptide BSN-37 which had a potential to replace antibiotics as new antimicrobial agents.

Key words: antibacterial peptide BSN-37; bacteriostatic activity; stability; physical and chemical properties; bactericidal dynamics

CLC Number:

S859.79⁺7

ZHANG Wei, HANG Bolin, SI Sujin, NING Chunmei, LI Jie, HU Jianhe. Bacteriostatic Activity and Stability Analysis of Antimicrobial Peptide BSN-37[J]. , 2019, 46(1): 287-295.

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Bacteriostatic Activity and Stability Analysis of Antimicrobial Peptide BSN-37

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