鹿角盘水提物对大鼠乳腺增生的作用效果及其机制研究

doi:10.16431/j.cnki.1671-7236.2019.01.035

Abstract

Abstract:

To explore the therapeutic effects of deer hard antler button (DHAB) water extracts on hyperplasia of mammary glands (HMG) in rats and to explore its mechanism of action and dosage,the model of HMG was established through intramuscular injection of estradiol benzoate (0.5 mg/(kg·d)) and progesterone (5.0 mg/(kg·d)) in 70 SD rats.There were 7 groups with 10 rats in each group,which were divided into blank group,HMG model group,positive drug tamoxifen group (0.036 mg/(kg·d)),4 DHAB groups (high-dose,H,2.625 g/(kg·d);Medium-high-dose,MH,1.575 g/(kg·d);Medium-low-dose,ML,1.05 g/(kg·d);Low-dose,L,0.63 g/(kg·d)).Each group was treated with intragastric administration for 45 d.The diameter and height of nipple and body weight were recorded weekly.Blood samples were taken and thymus,spleen,uterus and ovary weights of the rat breasts were measured at the end of the test.Histopathological sections and HE staining were performed and sex hormone estradiol (E₂) and progesterone (P) were detected by radioimmunoassay.The results indicated that different doses of DHAB water extracts could reduce the diameter and height of nipple,increase thymus and spleen indexes and reduce uterine index,reduce the number of breast lobules,acinar numbers and secretion contents,and decrease E₂ and increase P levels in serum of HMG rats.All these indexes were significantly or extremely significantly different from the model group (P < 0.01;P < 0.05).In conclusion,DHAB water extracts had significant therapeutic effects on HMG in rats,and the medium-high-dose was the best.The action mechanism of DHAB water extracts was enhancing immunity and regulating serum E₂ and P levels.

Key words: deer hard antler button(DHAB) water extracts; hyperplasia of mammary glands (HMG); therapeutic effect; mechanism of action

CLC Number:

S854.5

GUAN Qing, ZHAO Haiping, WANG Dongxu, WANG Yusu, LI Chunyi, XU Li. Effects of Deer Hard Antler Buttons Water Extracts on Hyperplasia of Mammary Glands in Rats and Underlying Mechanism[J]. , 2019, 46(1): 296-304.

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Effects of Deer Hard Antler Buttons Water Extracts on Hyperplasia of Mammary Glands in Rats and Underlying Mechanism

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