激素剂量、超排间隔及重复超排次数对和牛重复超排效果的影响

doi:10.16431/j.cnki.1671-7236.2021.09.031

Abstract

Abstract: The aim of this study was to investigate the effects of hormone dose, interval of superovulation and times of repeated superovulation on superovulation in Wagyu cattle, in order to provide evidence for establishing a stable and efficient repeated superovulation protocol. 63 Wagyu cattles aged 15-18-month-old were selected as superovulation donors. Among them, 17 donors were randomly divided into two groups, injected with a total dose of 200, 200, 200 mg FSH and a total dose of 200, 220, 240 mg FSH, respectively. Repeated superovulation was performed for 3 times with an interval of 30 days, to study the effect of hormone dosages on superovulation. 24 donors were randomly divided into three groups, each group was injected 200 mg dose of FSH for 3 consecutive times superovulation for 60, 45 and 30 days treated intervals, respectively, to study the effect of interval time of hormone injection on superovulation. 22 donors were injected with 200 mg FSH each time for 7 consecutive repetitions with an interval of 30 days, to study the effect of repetition times on superovulation. The results showed that the total number of embryos and the total number of available embryos obtained by the donors received 200, 220 and 240 mg doses of FSH were significantly higher than those obtained by the donors subjected to 200 mg dose of FSH treatment (P<0.05). By comparison with the superovulation interval of 60 days group, the number of available embryos produced at an interval of 45 days was significantly decreased (P<0.05), and the number of available embryos obtained by the donors in the 30 days interval group was also decreased, but there was no significant difference (P>0.05). There was no significant difference in the number of available embryos when repeated superovulation was less than 6 times (P>0.05). In summary, the effect of 200, 220 and 240 mg doses of FSH on repeated superovulation was better than that of 200, 200 and 200 mg dosage group. Longer superovulation interval was benefit to the recovery of reproductive tract and the available of available embryos, but the repetitious superovulation at 30 days interval could also get satisfactory results. The suitable number of superovulation times was 5 to 6 when the interval was 30 days for high intensive repeated superovulation.

Key words: Wagyu cattle; repeated superovulation; hormone dosage; superovulation interval; available embryos

CLC Number:

S823

WANG Lin, PANG Yunwei, HAO Haisheng, SONG Jinhui, ZHAO Shanjiang, DU Weihua, ZHAO Xueming, ZOU Huiying, ZHU Huabin. Effects of Hormone Dosage,Superovulation Interval and Repeated Times on Repeated Superovulation in Wagyu Cattle[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(9): 3394-3402.

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Effects of Hormone Dosage,Superovulation Interval and Repeated Times on Repeated Superovulation in Wagyu Cattle

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[1]	BI Jiang-hua, FENG Chun-tao, LI Su-xia, LIN Hui-liang, SUN Gui-lai, FANG Jin-wu, LI Shu-jing. Study on the Key Techniques of Rapid Propagation in Purebred Wagyu [J]. , 2017, 44(6): 1784-1789.
[2]	HAN Wei;YU Wen-li;LI Shu-jing;. Studies on Effect of High-intensity and Repeated Superovulation in Wagyu-catalo [J]. , 2012, 39(5): 170-173.
[3]	QUAN Fu-sheng;BAI Xue-yao;ZHAO Xiao-e;WANG Yong-sheng;HUA Song;ZHENG Yue-mao;ZHANG Yong. The Effect of FSH Dosage Adjustment on Boer Goat Repeated Superovulation [J]. , 2010, 37(7): 116-119.