水牛发情期唾液生殖激素和结晶与卵泡发育的关系

doi:10.16431/j.cnki.1671-7236.2021.02.021

Abstract

Abstract: The purpose of this study was to investigate the changes of reproductive hormones,salivary crystals and follicular development in buffalo saliva.The reproductive hormones in salivary and serum of water buffalo were measured by ELISA kit,salivary crystals were prepared,and the relationship between reproductive hormones and salivary crystals and follicular changes was analyzed.Four methods,including salivary crystallization,rectal examination,salivary crystallization+ B-ultrasound and rectal examination+B-ultrasound were used for verification in production.The results showed that the level of salivary estrogen (E₂) reached a peak of 233.51 pg/mL on the day of estrus,and then began to decrease slowly.The progesterone (P₄) level in buffalo saliva reached 16.17 ng/mL before 2 d of estrus,and decreased to 8.91 ng/mL on the estrus day.The level of follicle-stimulating hormone (FSH) gradually decreased from 37.91 ng/mL to 34.64 ng/mL before 2 d of estrus.However,it gradually increased to 61.20 ng/mL after 2 d of estrus.Luteinizing hormone (LH) gradually decreased from 5.15 ng/mL at 1 d before estrus to 4.12 ng/mL on the estrus day,but increased to 5.77 ng/mL at 1 d after estrus.B-ultrasonography results showed that ovarian follicles grew rapidly from 2 d before estrus to 20 mm on the day of estrus,and the size of follicles was insignificant until ovulation burst.After ovulation,the corpus luteum was formed and maintained until about 4 d before the next estrus,during which the corpus luteum was at its maximum at 14-17 d after estrus,which was consistent with the peak of LH secretion in buffalo saliva.The estrus rate determined by salivary crystallization+B-ultrasound was the highest (86.67%),which was significantly different from other methods (P<0.05).The results of pregnancy diagnosis showed that the pregnancy rate (61.53%) determined by salivary crystallization+B-ultrasound was significantly higher than that by rectal examination,salivary crystallography and rectal examination+B-ultrasound (P<0.05).To sum up,the development of follicles on ovaries was significantly related to the concentration of reproductive hormones in saliva.Saliva crystals on the day of estrus showed typical dendritic shape,and the accuracy of saliva crystals+B ultrasound method for estrus identification was the highest.

Key words: salivary crystallization; reproductive hormones; follicular development; buffaloes

CLC Number:

S823.9

JIA Yinhai, HUANG Mingguang, XU Wenwen, LI Fangfang, WEN Xinwang, LI Ming, YANG Xiurong, WU Zhuyue, JIANG Hesheng. Relationship Between Salivary Reproductive Hormones and Crystals with Follicular Development in Buffalo During Estrus[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(2): 592-598.

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Relationship Between Salivary Reproductive Hormones and Crystals with Follicular Development in Buffalo During Estrus

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