GnIH与INH表位多肽疫苗主动免疫对甘肃高山细毛羊生殖激素的影响

doi:10.16431/j.cnki.1671-7236.2016.04.028

Abstract

Abstract: This study aimed to detect the reproductive hormones levels of follicle-stimulating hormone (FSH) and luteinizing hormone (LH) in Gansu Alpine Fine-wool sheep by active immunization of two synthesized epitope peptide vaccines A and B which were combined inhibit hormone (INH) and gonadotropin inhibit hormone (GnIH).15 Gansu Alpine Fine-wool sheep were selected which were 3.5 year old and lived in anoestrus season, 3 groups each had 5 sheep, GnIH epitope peptide vaccine and INH epitope peptide vaccine were named epitope peptide vaccine A and B groups, while blank group was injected with saline.The blood was collected before immunization and immuned, and bloods were collected once every 7 days, meanwhile collected blood every 15 min after estrus.The serum was used to detect antibody, FSH and LH levels by ELISA Kits.The results showed that the epitope peptide vaccine A and B groups produced the corresponding antibodies after the initial immunization, and epitope peptide vaccine B group produced more antibodies than epitope peptide vaccine A group.Epitope peptide vaccine B group promoted the FSH secretion levels was significantly higher than epitope peptide vaccine A group in 75, 90 and 105 min (P<0.05);In 45 to 105 minutes, the LH secretion levels of epitope peptide vaccine B group was significantly higher than epitope peptide vaccine A group (P<0.05), while the LH and FSH levels in epitope peptide vaccine A and B groups were significantly higher than control group (P<0.05).This study indicated that the epitope peptide vaccine A and B groups could promote the FSH and LH secretion levels through active immunity in Gansu Alpine Fine-wool sheep and had a good immune function.This study laid a foundation for the application of epitope peptide vaccine on sheep.

Key words: gonadotropin inhibit hormone (GnIH); inhibit hormone (INH); active immune; Gansu Alpine Fine-wool sheep; reproductive hormones

CLC Number:

Q575⁺.12

ZHANG Ling-ling, YANG Bo-hui, YUE Yao-jing, GUO Ting-ting, LIU Jian-bin, YUAN Chao, NIU Chun-e, FENG Rui-lin, GUO Jian, SUN Xiao-ping, LIU Shan-bo. Effects of Active Immunization with GnIH and INH Epitope Peptide Vaccine on Reproductive Hormones of Gansu Alpine Fine-wool Sheep[J]. , 2016, 43(4): 1039-1044.

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Effects of Active Immunization with GnIH and INH Epitope Peptide Vaccine on Reproductive Hormones of Gansu Alpine Fine-wool Sheep

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