BMP4诱导的Smad9在小鼠卵泡发育中的作用及其机制的初步研究

doi:10.16431/j.cnki.1671-7236.2018.10.028

Abstract

Abstract:

This study was conducted to explore the role of Smad9 on follicular development in mice and provide new ideas for the study of follicular growth and its regulation mechanism.Six-week-old female Kunming mice were randomly divided into 3 groups and injected intraperitoneally with physiological saline,bone morphogenetic protein-4 (BMP4) and Smad9 inhibitor (LDN-193189),respectively,which were used as the control group,BMP4 group and LDN group.After 48 hours,the ovaries were collected to make paraffin sections.Follicles were observed after HE staining and the follicles at different stages of development were counted.Western blotting and Real-time PCR were used to detect Smad9 protein and mRNA levels.At the same time,the concentrations of estradiol (E₂),progesterone (P₄),luteinizing hormone (LH),follicle stimulating hormone (FSH) and aromatase in the serum of each group of mice were measured by ELISA,and the expression of genes such as CYP19a1,LHR,PRLR and FSHR were detected to further explore possible mechanisms of Smad9.The results showed that compared with control group,the Smad9 protein and mRNA levels were higher in BMP4 group and lower in LDN group;The number of antral follicles in BMP4 group was significantly increased (P < 0.05),while LDN group was significantly reduced (P < 0.05).In addition,ELISA results showed that the levels of serum E₂,FSH and aromatase increased,while the levels of P₄ and LH decreased in BMP4 group;The levels of E₂ and aromatase decreased in LDN group.The results of Real-time PCR showed that the mRNA levels of FSHR and CYP19a1 genes increased,while the mRNA levels of LHR and PRLR genes decreased in BMP4 group;The mRNA level of PRLR gene increased,while the mRNA level of CYP19a1 gene decreased in LDN group.From the above experimental results,it could be concluded that BMP4-induced Smad9 promoted antral follicles development,and it might work via affecting the production of E₂,PRL and aromatase.

Key words: BMP/Smads signaling pathway; Smad9; follicular development; mice

CLC Number:

S814

WANG Hejian, ZHANG Li, YU Daolun, CHEN Jie, XING Chaofeng, LI Jie, LI Jun, CAI Yafei. Preliminary Study on the Role of BMP4-induced Smad9 in Mouse Follicular Development and Its Mechanism[J]. , 2018, 45(10): 2902-2909.

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Preliminary Study on the Role of BMP4-induced Smad9 in Mouse Follicular Development and Its Mechanism

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