苗药对3种常见致病菌体外抑菌效果研究

doi:10.16431/j.cnki.1671-7236.2018.10.029

Abstract

Abstract:

To determine the antibacterial effect of 20 Miao national herbs such as Isatis tinctoria,gallnut and Astragalus and their prescriptions against Staphylococcus aureus (S.aureus),Salmonella and Escherichia coli (E.coli),the active ingredients of Miao herbal medicines were extracted using water decoction,ultrasonic water extraction and ultrasonic alcohol extraction,respectively.Double dilution method was used to assess the minimal inhibitory concentration (MIC) for S.aureus,Salmonella and E.coli.The results showed that antibacterial effect of Miao national single herbs in three different extraction methods was ultrasonic alcohol extraction method > ultrasonic water extraction method > water decoction method.The MIC of the ethanol extracts of gallnut and Agrimonia on S.aureus,Salmonella and E.coli were 0.18,0.71 and 1.43 mg/mL (gallnut) and 1.56,3.13 and 3.13 mg/mL (Agrimonia),respectively.Furthermore,the MIC of Kadsura,plum,thorn pear,Lonicera,Rhizoma Dryopteris Crassirhizomae and Eucommia were higher than gallnut and Agrimonia,but lower than other 12 kinds of Miao national single herbs.The MIC of these 6 kinds of Miao national single herbs extracts extracted in ultrasonic alcohol against S.aureus,Salmonella and E.coli were 3.69 to 50.00,3.13 to 50.00 and 3.31 to 66.25 mg/mL,respectively.The effect of prescriptions of Miao national herbs showed that ultrasonic water extraction method > ultrasonic alcohol extraction method > water decoction method.The MIC of prescription 5 ultrasonic water extract was the lowest for S.aureus,Salmonella and E.coli,which were 0.34,2.75 and 2.75 mg/mL,respectively.The MIC of the ultrasonic water extract of prescription 1 against S.aureus,Salmonella and E.coli was lower than the prescriptions 2,3 and 4,which were 2.88,5.75 and 5.75 mg/mL,respectively.The results showed that gallnut,Agrimonia and prescription 5 had strong antibacterial activity against 3 common pathogens,Kadsura,plum,thorn pear,Lonicera,Rhizoma Dryopteris Crassirhizomae,Eucommia and prescription 1 had certain antibacterial effects,and the antibacterial effects of other Miao drugs were relatively weak.

Key words: Miao herbal medicine; S.aureus; Salmonella; E.coli; bacteriostasis in vitro

CLC Number:

S853.7

YANG Linhua, CHEN Shengchang, QIAO Yanlong, XIN Hailiang, WANG Qingfeng, WANG Fu, KOU Hong, LU Xiaoluo, ZHANG Taotao. Bacteriostasis in vitro of Miao National Herbs Against Three Common Pathogens[J]. , 2018, 45(10): 2910-2917.

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Bacteriostasis in vitro of Miao National Herbs Against Three Common Pathogens

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