水牛发情周期生殖激素变化规律及唾液结晶的分析

doi:10.16431/j.cnki.1671-7236.2019.04.017

Abstract

Abstract:

The aim of the experiment was to explore the buffalo estrus and provide theoretical references for buffalo production by studying the change laws of estradiol (E₂) and progesterone (P₄) concentrations during estrus cycle of salivary and serum,investigating the salivary ferning and the development of ovaries in buffalo.The concentrations of E₂ and P₄ were determined by the enzyme linked immunosorbent assay (ELISA) in estrus cycle,and the correlation between the serum and saliva hormones were analyzed.The results show that the concentrations of E₂ and P₄ varied fluctuantly.The concentration of saliva P₄ was maintained from 6.50 to 7.10 ng/mL at the beginning of estrus,and reached 11.09 ng/mL at 13 d of estrus,then fell fast.The concentration of saliva E₂ had two peaks,respectively at 3-5 d (178.53 pg/mL) and 14-17 d (179.10 pg/mL) of estrus.The change trends of E₂ and P₄ levels in salivary and serum had the same change trends,the concentrations of salivary P₄ and E₂ had significant correlations with that in serum (P<0.05),while the salivary E₂ concentration also had a extremely significant correlation with the salivary P₄ concentration (P<0.01).Saliva showed a typical fern-like crystallization patterns at 0 d of estrus,with significantly lower fractal dimension values compared with the other days of estrus (P<0.05).The results were also showed the basic synchronization between saliva crystallization and ovarian follicle.In conclusion,saliva crystallization could be used as a reliable index for monitoring estrus and predicting ovulation in buffalo.

Key words: buffalo; reproduction hormones; salivary crystallization

CLC Number:

S814.1

JIA Yinhai, LI Fangfang, JIANG Shiqiang, LI Ming, XU Wenwen, WANG Guoli, PAN Nengqing, YANG Xiurong, JIANG Hesheng. The Variations and Correlation Analysis of Saliva Reproduction Hormones and Crystallization Patterns During Estrus Cycle in Buffaloes[J]. , 2019, 46(4): 1101-1107.

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The Variations and Correlation Analysis of Saliva Reproduction Hormones and Crystallization Patterns During Estrus Cycle in Buffaloes

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