绵羊卵泡发育过程中关键信号通路研究进展

doi:10.16431/j.cnki.1671-7236.2021.03.020

Abstract

Abstract: The entire development process of a follicle is precisely regulated,which includes primary follicles to mature follicles,ovulation and corpus luteum development.It is critical to produce large quantity of superior follicles for multiple births and rapid reproduction.It was found that through impacting the growth of oocyte and granulosa cells in sheep follicles,the associated signal pathways and transcription factors in turn regulate the growth and maturity of follicles.Further understanding of these signal pathways is useful for investigating the regulatory mechanism of follicular development.In addition,high efficiency of sheep breeding and reproduction can be achieved as soon as possible.Notch is a highly conservative signaling pathway that plays an important role in the development of follicles.The PI3K/AKT/mTOR signaling pathway is widely present in cells.Each member of this pathway is a signal transduction molecule and has a huge impact in the early stage of follicular development.Physical junctions such as gap junction (GJ) and transzonal projections (TZPs) play an important role in signal communication between cells.This article elaborated the regulatory function of Notch signaling pathway,PI3K/AKT/mTOR signaling pathway,gap junctions and transzonal projections in the development of sheep follicles,which would provide a reference for further exploring the regulatory mechanism of sheep follicle development.

Key words: sheep; follicular development; Notch signaling pathway; PI3K/AKT/mTOR signaling pathway; gap junction; transzonal projections

CLC Number:

S826

CHEN Xin, DA Lai, FU Shaoyin, QI Yunxia, WANG Biao, HE Xiaolong, MA Yuejun, FANG Qinyuan, ZHANG Lin, JIA Mingrui, DONG Peng, LIU Yongbin. Advances on Critical Signaling Pathway in the Development of Sheep Follicles[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(3): 963-974.

References

[1] VITT U A,HAYASHI M,KLEIN C.Growth differentiation factor-9 stimulates proliferation but suppresses the follicle stimulating hormone induced differentiation of cultured granulosa cells from small antral and preovulatory rat follicles[J].Biology of Reproduction,2000,62(2):370-377.
[2] BROWER P T,SCHULTZ R M.Intercellular communication between granulosa cells and mouse oocytes:Existence and possible nutritional role during oocyte growth[J].Developmental Biology,1982,90(1):144-153.
[3] HSUEH A J W,KAWAMURA K,CHENG Y,et al.Intraovarian control of early folliculogenesis[J].Endocrine Reviews,2015,36(1):1-24.
[4] 吴玉萍,赵茴茴,周玉霞,等.颗粒细胞在卵母细胞发育成熟中的作用[J].国际生殖健康/计划生育杂志,2017,36(6):503-506. WU Y P,ZHAO H H,ZHOU Y X,et al.The role of granulosa cells in oocyte maturation[J].Journal of International Reproductive Health/Family Planning,2017,36(6):503-506.(in Chinese)
[5] MOHR O L.Character changes caused by mutation of an entire region of a chromosome in Drosophila[J].Genetics,1919,4(3):275-282.
[6] 刘洋,田见晖,王淑敏,等.Notch信号通路与哺乳动物卵泡发育的研究进展[J].黑龙江畜牧兽医,2015,21:79-82. LIU Y,TIAN J H,WANG S M,et al.Research progress of Notch signaling pathway and mammalian follicular development[J].Heilongjiang Animal Science and Veterinary Medicine,2015,21:79-82.(in Chinese)
[7] 孙梦黎.Notch信号通路在富集卵巢生殖干细胞的卵巢上皮组织和卵巢生殖干细胞的表达及动态变化[D].南昌:南昌大学,2014. SUN M L.The expression and dynamic changes of Notch signaling pathway in ovarian epithelial where enrich ovarian germline stem cells and ovarian germline stem cells[D].Nanchang:Nanchang University,2014.(in Chinese)
[8] 雷俊川.Notch信号途径在哺乳动物卵泡发育中作用的初步研究[D].西安:第四军医大学,2008. LEI J C.Preliminary study of effect of Notch signaling on the ovarian follicular development in the mammals[D].Xi'an:Fourth Military Medical University,2008.(in Chinese)
[9] ARRUGA F,VAISITTI T,DEAGLIO S.The Notch pathway and its mutations in mature B cell malignancies[J].Frontiers in Oncology,2018,8(8):550-567.
[10] VANORNY D A,MAYO K E.The role of Notch signaling in the mammalian ovary[J].Reproduction,2017,153(6):187-204.
[11] JOHNSON J,ESPINOZA T,MCGAUGHEY R W,et al.Notch pathway genes are expressed in mammalian ovarian follicles[J].Mechanisms of Development,2001,109(2):355-361.
[12] DORFMAN M D,KERR B,GARCIA-RUDAZ C,et al.Neurotrophins acting via TRKB receptors activate the JAGGED1-NOTCH2 cell-cell communi-cation pathway to facilitate early ovarian develop-ment[J].Endocrinology,2011,152(12):5005-5016.
[13] JING J,JIANG X,CHEN J,et al.Notch signaling pathway promotes the development of ovine ovarian follicular granulosa cells[J].Animal Reproduction Science,2017,181(3):69-78.
[14] VANORNY D A,PRASASYA R D,CHALPE A J,et al.Notch signaling regulates ovarian follicle formation and coordinates follicular growth[J].Molecular Endocrinology,2014,28(4):499-511.
[15] 黄堃.CAV1和JAK在原始卵泡形成中的功能与机制研究[D].北京:中国农业大学,2018. HUANG K.Functions and mechanisms of CAV1 and JAK in primordial follicle formation[D].Beijing:China Agricultural University,2018.(in Chinese)
[16] LIU T E,WANG S,ZHANG L,et al.Growth hormone treatment of premature ovarian failure in a mouse model via stimulation of the Notch1 signaling pathway[J].Experimental and Therapeutic Medicine,2016,12(1):215-221.
[17] WANG L Q,LIU J C,CHEN C L.Regulation of primordial follicle recruitment by cross talk between the Notch and phosphatase and tensin homologue (PTEN)/AKT pathways[J].Reproduction,Fertility and Development,2016,28(6):700-712.
[18] 姜晓龙.DAPT阻断Notch信号通路对绵羊卵泡颗粒细胞功能的影响[D].太谷:山西农业大学,2015. JIANG X L.Effect of Notch signling pathway blocked with DAPT on function of sheep granulosa cells in vitro[D].Taigu:Shanxi Agricultural University,2015.(in Chinese)
[19] JOHNSON J,ESPINOZA T,MCGAUGHEY R W,et al.Notch pathway genes are expressed in mammalian ovarian follicles[J].Mechanisms of Development,2001,109(2):355-361.
[20] FENG Y M,LIANG G J,PAN B,et al.Notch pathway regulates female germ cell meiosis progression and early oogenesis events in fetal mouse[J].Cell Cycle,2014,13(5):782-791.
[21] WANG Z,NIU W,WANG Y,et al.Follistatin288 regulates germ cell cyst breakdown and primordial follicle assembly in the mouse ovary[J].PLoS One,2015,10(6):e0129643.
[22] FENG L,WANG Y,CAI H,et al.ADAM10-Notch signaling governs the recruitment of ovarian pregranulosa cells and controls folliculogenesis in mice[J].Journal of Cell Science,2016,129(11):2202-2212.
[23] ZHANG C P,YANG J L,ZHANG J,et al.Notch signaling is involved in ovarian follicle development by regulating granulosa cell proliferation[J].Endocrino-logy,2011,152(6):2437-2447.
[24] 王静,包日强,董麒铭,等.Notch信号通路对卵母细胞发育的影响[J].南昌大学学报(理科版),2015,39(4):393-397. WANG J,BAO R Q,DONG Q M,et al.The influence of Notch signling on oocyte development[J].Journal of Nanchang University (Natural Science),2015,39(4):393-397.(in Chinese)
[25] MANOSALVA I,GONZ A,KAGEYAMA R.Hes1 in the somatic cells of the murine ovary is necessary for oocyte survival and maturation[J].Developmental Biology,2013,375(2):140-151.
[26] MURTA D,BATISTA M,SILVA E,et al.Differential expression of Notch component and effector genes during ovarian follicle and corpus luteum development during the oestrous cycle[J].Reproduction,Fertility and Development,2015,27(5):1038-1048.
[27] PRASASYA R D,MAYO K E.Notch signaling regulates differentiation and steroidogenesis in female mouse ovarian granulosa cells[J].Endocrinology,2018,159(1):184-198.
[28] MATSUOKA T,YASHIRO M.The role of PI3K/Akt/mTOR signaling in gastric carcinoma[J].Cancers,2014,6(3):1441-1463.
[29] 吕西.蜂斗菜素通过PI3K-Akt-mTOR信号通路抗结肠癌作用体外和体内实验研究[D].兰州:兰州大学,2019. LYU X.Involvement of PI3K-Akt-mTOR signaling pathway in anti-colon carcinoma effects ofpetasin in vitro and in vivo[D].Lanzhou:Lanzhou University,2019.(in Chinese)
[30] 李鑫峰.苏木提取物对PI3K/Akt/mTOR信号通路介导的细胞自噬的影响[D].哈尔滨:黑龙江中医药大学,2019. LI X F.Effects of sappan extract on autophagy mediated via PI3K/Akt/mTOR signaling pathway[D].Harbin:Heilongjiang University of Chinese Medicine,2019.(in Chinese)
[31] KAPLAN D R,WHITMAN M,SCHAFFHAUSEN B,et al.Common elements in growth factor stimulation and oncogenic transformation:85 kd phosphoprotein and phosphatidylinositol kinase activity[J].Cell,1987,50(7):1021-1029.
[32] 邹鹏达.LH诱导山羊卵泡膜细胞Akt磷酸化及对雄激素生成的影响[D].杨陵:西北农林科技大学,2016. ZOU P D.Luteinizing hormone-induced Akt phosphorylation and its effect on androgen production in goat theca cells[D].Yangling:Northwest A&F University,2016.(in Chinese)
[33] 尹承龙,劳学军.PI3K-AKT-mTOR信号通路的研究进展[J].中国医学创新,2016,13(1):145-148. YIN C L,LAO X J.Research progress on PI3K-AKT-mTOR signaling pathway[J].Medical Innovation of China,2016,13(1):145-148.(in Chinese)
[34] 刘洋.Notch信号通路相关基因在牛卵泡发育中表达情况的初步研究[D].长春:吉林农业大学,2015. LIU Y.A preliminary study of notch signaling pathway genes expression in bovine ovarian follicle development[D].Changchun:Jilin Agricultural University,2015.(in Chinese)
[35] WULLSCHLEGER S,LOEWITH R,HALL M N.mTOR signaling in growth and metabolism[J].Cell,2006,124(3):471-484.
[36] XIA H,ZHANG R,GUAN H,et al.Follicle loss and PTEN/PI3K/mTOR signaling pathway activated in LepR-mutated mice[J].Gynecological Endocrinology,2019,35(1):44-48.
[37] JUNG C H,RO S H,CAO J,et al.mTOR regulation of autophagy[J].FEBS Letters,2010,584(7):1287-1295.
[38] DADA S,DEMANINES N,DORMOND O.mTORC2 regulates PGE2-mediated endothelial cell survival and migration[J].Biochemical and Biophysical Research Communications,2008,372(4):875-879.
[39] 辛晓萍,袁晓龙,陈炫,等.PI3K信号通路关键基因在猪卵泡发育中的表达规律[J].华南农业大学学报,2018,39(2):23-28. XIN X P,YUAN X L,CHEN X,et al.Expression patterns of PI3K signaling pathway key genes in porcine follicular development[J].Journal of South China Agricultural University,2018,39(2):23-28.(in Chinese)
[40] 姜凤丽,王晓滨,宗婧,等.PI3K-Akt/mTOR信号通路对卵巢早衰相关性研究进展[J].黑龙江科学,2019,10(2):50-51. JIANG F L,WANG X B,ZONG J,et al.Research progress on the correlation between PI3K-Akt/mTOR signaling pathway and premature ovarian failure[J].Heilongjiang Science,2019,10(2):50-51.(in Chinese)
[41] SAXTON R A,SABATINI D M.mTOR signaling in growth,metabolism,and disease[J].Cell,2017,168(6):960-976.
[42] 綦松智,吴登俊,张中显.mTOR对信号通路调控的研究进展[J].中国畜牧杂志,2010,46(1):57-60. QI S Z,WU D J,ZHANG Z X.Advances in mTOR on signaling pathway[J].Chinese Journal of Animal Science,2010,46(1):57-60.(in Chinese)
[43] 代从新,刘小海,孙博文,等.PI3K/AKT/mTOR信号通路在垂体腺瘤中的研究进展[J].中华医学杂志,2014,94(9):717-718. DAI C X,LIU X H,SUN B W,et al.Research progress on PI3K/AKT/mTOR signaling pathway in pituitary adenomas[J].National Medical Journal of China,2014,94(9):717-718.(in Chinese)
[44] 郜然然.补肾养血活血法经PI3K/AKT/mTOR信号通路调控凋亡及自噬流促卵泡发育的分子机制[D].成都:成都中医药大学,2019. GAO R R.The molecular mechanism of tonifying kidney,nourishing and activating blood therapy in regulating apoptosis and autophagy flux through PI3K/AKT/mTOR signaling pathway[D].Chengdu:Chengdu University of Traditional Chinese Medicine,2019.(in Chinese)
[45] 白俊.基于PI3K/AKT/mTOR信号通路研究补肾活血法调控大鼠卵巢颗粒细胞自噬的分子机制[D].成都:成都中医药大学,2018. BAI J.Study on the molecular mechanism of nourishing kidney and activating blood therapy in regulating granulosa cells autophagy of rat ovary based on PI3K/AKT/mTOR signaling pathway[D].Chengdu:Chengdu University of Traditional Chinese Medicine,2018.(in Chinese)
[46] BEZERRA M S,BARBERINO R S,MENEZES V G,et al.Insulin-like growth factor-1(IGF-1) promotes primordial follicle growth and reduces DNA fragmentation through the phosphatidylinositol 3-kinase protein kinase B (PI3K AKT) signalling pathway[J].Reproduction,Fertility and Development,2018,30(7):1503-1513.
[47] SANTOS J M S,MONTE A P O,LINS T L B G,et al.Kaempferol can be used as the single antioxidant in the in vitro culture medium,stimulating sheep secondary follicle development through the phosphatidyli-nositol 3-kinase signaling pathway[J].Theriogenology,2019,136(4):86-94.
[48] SANTOS J M S,LINS T L B G,BARBERINO R S,et al.Kaempferol promotes primordial follicle activation through the phosphatidylinositol 3-kinase protein kinase B signaling pathway and reduces DNA fragmentation of sheep preantral follicles cultured in vitro[J].Molecular Reproduction and Development,2019,86(5):319-329.
[49] MACEDO T J S,BARROS V R P,MONTE A P O,et al.Resveratrol has dose-dependent effects on DNA fragmentation and mitochondrial activity of ovine secondary follicles cultured in vitro[J].Zygote,2017,25(4):434-442.
[50] 王凤伟,王静芬,徐键.参与调控始基卵泡激发过程的细胞信号通路[J].生殖与避孕,2016,36(3):208-213. WANG F W,WANG J F,XU J.Cell signaling pathways involved in the regulation of primordial follicle stimulation[J].Chinese Journal of Reproduction and Contraception,2016,36(3):208-213.(in Chinese)
[51] ZHAO Y,ZHANG Y,LI J,et al.MAPK3/1 participates in the activation of primordial follicles through mTORC1-KITL signaling[J].Journal of Cellular Physiology,2017,233(1):226-237.
[52] 杨静,梁嘉丽,秦佳佳.PI3K/Akt信号通路与卵巢早衰相关性的研究进展[J].现代妇产科进展,2016,25(2):156-158. YANG J,LIANG J L,QIN J J.Research progress on the relationship between PI3K/AKT signaling pathway and premature ovarian failure[J].Progress in Obstetrics and Gynecology,2016,25(2):156-158.(in Chinese)
[53] 李倩.PI3K p110delta催化亚基在小鼠卵巢卵泡生长中的功能研究[D].南京:南京医科大学,2013. LI Q.The p110delta isoform of phosphoinositide 3-kinase catalytic subunits is key for hormonally stimulated ovarian follicle growth[D].Nanjing:Nanjing Medical University,2013.(in Chinese)
[54] SHEN M,JIANG Y,GUAN Z,et al.Protective mechanism of FSH against oxidative damage in mouse ovarian granulosa cells by repressing autophagy[J].Autophagy,2017,13(8):1364-1385.
[55] 李晓雯,郎朗,季宇彬,等.卵泡刺激素促进卵巢颗粒细胞增殖分化的信号通路的研究进展[J].北京联合大学学报,2012,26(4):46-50. LI X W,LANG L,JI Y B,et al.Study on process of the signaling pathway of follicle stimulating hormone to promote the ovarian granulosa cell proliferation and differentiation[J].Journal of Beijing Union University,2012,26(4):46-50.(in Chinese)
[56] 曹婉宁,董晓英.mTOR通路参与卵泡生长发育相关机制的研究进展[J].生殖医学杂志,2016,25(5):469-472. CAO W N,DONG X Y.Research progress of mechanism of mTOR pathway involves follicular development[J].Journal of Reproductive Medicine,2016,25(5):469-472.(in Chinese)
[57] ADIB S,VALOJERDI M R,ALIKHANI M.Dose optimisation of PTEN inhibitor,bpV (HOpic),and SCF for the in vitro activation of sheep primordial follicles[J].Growth Factors,2019,37(2):178-189.
[58] GROSBOIS J,DEMEESTERE I.Dynamics of PI3K and Hippo signaling pathways during in vitro human follicle activation[J].Human Reproduction,2018,33(6):1705-1714.
[59] WANG W,YIN L,BAI L,et al.Bmal1 interference impairs hormone synthesis and promotes apoptosis in porcine granulosa cells[J].Theriogenology,2017,99(4):63-68.
[60] WEN R,HU S,XIAO Q,et al.Leptin exerts proliferative and anti-apoptotic effects on goose granulosa cells through the PI3K/AKT/mTOR signaling pathway[J].The Journal of Steroid Biochemistry and Molecular Biology,2015,149(9):70-79.
[61] MAKKER A,GOEL M M,MAHDI A A.PI3K/PTEN/Akt and TSC/mTOR signaling pathways,ovarian dysfunction,and infertility:An update[J].Journal of Molecular Endocrinology,2014,53(3):103-118.
[62] SOBINOFF A P,NIXON B,ROMAN S D,et al.Staying alive:PI3K pathway promotes primordial follicle activation and survival in response to 3MC-induced ovotoxicity[J].Toxicological Sciences,2012,128(1):258-271.
[63] GRAZUL-BILSKA A T,REDMER D A,BILSKI J J,et al.Gap junctional proteins,connexin 26,32,and 43 in sheep ovaries throughout the estrous cycle[J].Endocrine,1998,8(3):269-280.
[64] 赵杰,陈秀娟.颗粒细胞与卵母细胞关系研究进展[J].国际生殖健康/计划生育杂志,2015,34(5):406-409. ZHAO J,CHEN X J.Relationship between granular cell and oocyte[J].Journal of International Reproduc-tive Health/Family Planning,2015,34(5):406-409.(in Chinese)
[65] GERSHON E,PLAKS V,DEKEL N.Gap junctions in the ovary:Expression,localization and function[J].Molecular and Cellular Endocrinology,2008,282(1-2):18-25.
[66] 张辉.雌激素膜受体GPR30在山羊卵丘卵母细胞复合体间隙连接通讯中的作用及其机制研究[D].杨陵:西北农林科技大学,2018. ZHANG H.Effect of estrogen membrane receptor GPR30 on gap junctional communicationof goat cummulus oocyte complex and its mechanism[D].Yangling:Northwest A&F University,2018.(in Chinese)
[67] 陈建伟.Notch2基因在绵羊卵巢表达的研究[D].晋中:山西农业大学,2015. CHEN J W.Study on Notch2 gene expression in sheep ovarie[D].Jinzhong:Shanxi Agricultural University,2015.(in Chinese)
[68] DELVAEYE T,VANDENABEELE P,BULTYNCK G,et al.Therapeutic targeting of connexin channels:New views and challenges[J].Trends in Molecular Medicine,2018,24(3):1036-1053.
[69] NUTTINCK F,PEYNOT N,HUMBLOT P,et al.Comparative immunohistochemical distribution of connexin 37 and connexin 43 throughout folliculogenesis in the bovine ovary[J]. Molecular Reproduction and Development,2000,57(1):60-66.
[70] KUNG F T,CHANG S Y,YANG C Y,et al.Transfer of nonselected transferable day 3 embryos in low embryo producers[J].Fertility and Sterility,2003,80(6):1364-1370.
[71] GITTENS J E I,KIDDER G M.Differential contributions of connexin37 and connexin43 to oogenesis revealed in chimeric reaggregated mouse ovaries[J].Journal of Cell Science,2005,118(1):5071-5078.
[72] BOROWCZYK E,JOHNSON M L,BILSKI J J,et al.Gap junctional connexin 37 is expressed in sheep ovaries[J].Endocrine,2006,30(2):223-230.
[73] BOROWCZYK E,JOHNSON M L,BILSKI J J,et al.Role of gap junctions in regulation of progesterone secretion by ovine luteal cells in vitro[J].Reproduction,2007,133(3):641-651.
[74] PRAVEEN V,CHAKRAVARTHI S S R,KONAA V N,et al.Quantitative patterns of expression of gap junction genes during in vivo or in vitro development of ovarian follicles in sheep[J].Small Ruminant Research,2016,143(8):35-42.
[75] SADOWSKA G B,STOPA E G,STONESTREET B S.Ontogeny of connexin 32 and 43 expression in the cerebral cortices of ovine fetuses,newborns,and adults[J].Brain Research,2009,1255(7):51-56.
[76] MAKITA M,MIYANO T.Steroid hormones promote bovine oocyte growth and connection with granulosa cells[J].Theriogenology,2014,82(4):605-612.
[77] ANDERSON E,ALBERTINI D F.Gap junctions between the oocyte and companion follicle cells 350 in the mammalian ovary[J].Journal of Cell Biology,1976,71(2):680-686.
[78] MOTTA P M,MAKABE S,NAGURO T,et al.Oocyte follicle cells association during development of human ovarian follicle.A study by high resolution scanning and transmission electron microscopy[J].Archives of Histology and Cytology,1994,57(4):369-394.
[79] ZUCCOTTI M,MERICO V,CECCONI S,et al.What does ittake to make a developmentally competent mammalian egg?[J].Human Reproduction Update,2011,17(4):525-540.
[80] BROWER P T,SCHULTZ R M.Intercellular communication between granulosa cells and mouse oocytes:Existence and possible nutritional role during oocyte growth[J].Developmental Biology,1982,90(1):144-153.
[81] ANDERSON E,ALBERTINI D F.Gap junctions between the oocyte and companion follicle cells in the mammalian ovary[J].Journal of Cell Biology,1976,71(2):680-686.
[82] ELHAYEK S,YANG Q,ABBASSI L,et al.Mammalian oocytes locally remodel follicular architecture to provide the foundation for germline-soma communication[J].Current Biology,2018,28(7):1124-1131.
[83] LOWTHER K M,FAVERO F,YANG C R,et al.Embryonic poly(A)-binding protein is required at the preantral stage of mouse folliculogenesis for oocyte-somatic communication[J].Biology of Repro-duction,2017,96(2):341-351.
[84] WASSARMAN P M,ALBERTINI D F.The mammalian ovum[J].Physiology of Reproduction,1994,11(3):79-122.
[85] YIN C,LIU J,HE B,et al.Heat stress induces distinct responses in porcine cumulus cells and oocytes associated with disrupted gap junction and trans-zonal projection colocalization[J].Journal of Cellular Physiology,2019,234(6):4787-4798.

Advances on Critical Signaling Pathway in the Development of Sheep Follicles

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