影响意大利水牛精液品质的相关因素分析

doi:10.16431/j.cnki.1671-7236.2020.09.024

Abstract

Abstract: This study was aimed to investigate the factors affecting semen quality by analyzing the semen quality of Italian buffaloes,measuring the circumference of the testis,the level of oxidative stress in seminal plasma and the expression of genes related to sperm motility.In this study,the sperm vitality,deformity rate and semen collection of 6 Italian buffaloes were tested,and the correlation analysis was carried out after measuring scrotal circumference.The oxidative stress level of seminal plasma in Italian buffaloes was tested,including malondialdehyde (MDA),total superoxide dismutase (T-SOD) and glutathione peroxidation (GSH-Px).The expression of β-tublin-2c (TUBB2C),outer dense fibres 2 (ODF2),tektin-2 (TEKT2),tektin-4 (TEKT4) genes and their correlation with sperm in Italian buffaloes were analyzed.The results showed that the correlation between scrotal circumference and semen yield,vigor and deformity rate was found,the coefficients were 0.423 (P>0.05),0.750 (P<0.01) and -0.827 (P<0.01),which showed a significant positive correlation between scrotal circumference and sperm motility,and a significant negative correlation with the deformity rate.The oxidative stress level in seminal plasma of Italian buffaloes was tested,the correlation coefficients of MDA,T-SOD and GSH-Px with sperm motility were -0.522 (P<0.05),0.333 (P>0.05) and 0.474 (P<0.05),respectively.There was a significant negative correlation between sperm motility and MDA content,a significant positive correlation between GSH-Px activity and sperm motility,no significant correlation with T-SOD activity.The correlation coefficients between sperm motility and TUBB2C,ODF2,TEKT2 and TEKT4 genes were 0.930 (P<0.01),0.726 (P<0.01),0.924(P<0.01) and 0.839 (P<0.01),respectively.There was a significant positive correlation between sperm motility and the expression of four genes.The results provided a reference for understanding the factors affecting the semen quality of Italian buffaloes,and also provided a theoretical basis for the selection of Italian bull.

Key words: Italian buffaloes; semen quality; scrotal circumference; oxidative stress; gene; correlation

CLC Number:

S814.8

CUI Zhichao, TANG Long, SUN Le, HUANG Shihai, XUE Qingsong, YANG Ting, SHI Deshun, LI Xiangping. Analysis of Related Factors in Semen Quality of Italian Buffaloes[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(9): 2899-2905.

References

[1] 王维勇,龚婷,王婷婷,等.从江香猪生精上皮周期及睾丸发育的形态学分析[J].中国畜牧兽医,2019,46(7):2012-2020. WANG W Y,GONG T,WANG T T,et al.Morphological analysis of spermatogenic epithelial cycle and testicular development in Congjiang Xiang pig[J].China Animal Husbandry & Veterinary Medicine,2019,46(7):2012-2020.(in Chinese)
[2] SOARES A,GUIMARAES S,KELLY M J,et al.Multiple-trait genome wide mapping and gene network analysis for scrotal circumference growth curves in Brahman cattle[J].Journal of Animal Science,2017,95(8):3331-3345.
[3] BUCAK M N,TUNCER P B,SARIOZKAN S,et al.Effects of antioxidants on post-thawed bovine sperm and oxidative stress parameters:Antioxidants protect DNA integrity against cryodamage[J].Cryobiology,2010,61(3):248-253.
[4] 董亮,何永志,王远亮,等.超氧化物歧化酶(SOD)的应用研究进展[J].中国农业科技导报,2013,15(5):53-58. DONG L,HE Y Z,WANG Y L,et al.Application of superoxide dismutase (SOD)[J].Chinese Journal of Agricultural Science and Technology,2013,15(5):53-58.(in Chinese)
[5] 平措占堆.奶牛精液品质性状的分子标记技术研究进展[J].中国奶牛,2018,1:15-19. PING C Z D.Advances in molecular marker technology for semen quality traits of dairy cows[J].Chinese Dairy Cows,2018,1:15-19.(in Chinese)
[6] PANG Y,YAN J,AN B,et al.The synthesis and evaluation of new butadiene derivatives as tubulin polymerization inhibitors[J].Bioorganic and Medicinal Chemistry,2017,25(12):3059-3067.
[7] YAMAGUCHI A,KANEKO T,INAI T,et al.Molecular cloning and subcellular localization of Tektin 2-binding protein 1(Ccdc172) in rat spermatozoa[J].Journal of Histochemistry and Cytochemistry,2014,62(4):286-297.
[8] XU M,ZHOU Z,CHENG C,et al.Cloning and characterization of a novel human TEKTIN1 gene[J].The International Journal of Biochemistry & Cell Biology,2001,33(12):1172-1182.
[9] WOLKOWICZ M J,NAABY-HANSEN S,GAMBLE A R,et al.Tektin B1 demonstrates flagellar localization in human sperm[J].Biology of Reproduction,2002,66(1):241-250.
[10] BHILAWADIKAR R,ZAVERI K,MUKADAM L,et al.Levels of Tektin 2 and CatSper 2 in normozoospermic and oligoasthenozoospermic men and its association with motility,fertilization rate,embryo quality and pregnancy rate[J].Journal of Assisted Reproduction and Genetics,2013,30(4):513-523.
[11] KANEKO T,MURAYAMA E,KURIO H,et al.Characterization of spetex-1,a new component of satellite fibrils associated with outer dense fibers in the middle piece of rodent sperm flagella[J].Molecular Reproduction and Development,2010,77(4):363-372.
[12] THEPPARAT T,KATAWATIN S,VONGPRALUB T,et al.Separation of bovine spermatozoa proteins using 2D-PAGE revealed the relationship between tektin-4 expression patterns and spermatozoa motility[J].Theriogenology,2012,77(9):1816-1821.
[13] COOPER T G.Looking forward to human sperm morphology[J].Human Reproduction,2016,31(1):8-9.
[14] KUMBHAR U B,GULAVANE S U,GAIKWAD S M,et al.Correlation of testicular ultrasonography,testicular biometry,serum testosterone levels and seminal attributes in pre and post-pubertal age for breeding soundness evaluation in Osmanabadi bucks[J].Tropical Animal Health and Production,2019,51(6):1467-1480.
[15] DANG Y H,ZHILAN L I,PAN L,et al.Effect of acrylonitrile induced reactive oxygen species generated on sperm quality and flagella ultrastructure in rats[J].Journal of Toxicology,2017,4(31):258-262.
[16] DOROSTGHOAL M,KAZEMINEJAD S R,SHAHBAZIAN N,et al.Oxidative stress status and sperm DNA fragmentation in fertile and infertile men[J].Andrologia,2017,49(10):e12762.
[17] GRANT K E,DE OLIVEIRA R V,HENNINGTON B S,et al.Sperm superoxide dismutase is associated with bull fertility[J].Reproduction Fertility and Development,2015,28(9):1405-1413.
[18] ZHAO W,LI Z,PING P,et al.Outer dense fibers stabilize the axoneme to maintain sperm motility[J].Journal of Cellular and Molecular Medicine,2017,22(3):1755-1768.
[19] XIONG Z C,ZHANG H H,HUANG B,et al.Expression pattern of prohibitin,capping actin protein of muscle Z-line beta subunit beta and tektin-2 gene in Murrah buffalo sperm and its relation-ship with sperm motility[J].Asian-Australasian Journal of Animal Sciences,2018,31(11):1729-1737.
[20] ALSHAWA E,LAQQAN M,MONTENARH M,et al.Influence of cryopreservation on the CATSPER2 and TEKT2 expression levels and protein levels in human spermatozoa[J].Toxicology Reports,2019,6:819-824.
[21] GAO K,WANG Z Q,LIU X C,et al.Genetic genes associated with oligospermia,asthenospermia and teratospermia:Advances in studies[J].National Journal of Andrology,2017,23(4):367-371.

Analysis of Related Factors in Semen Quality of Italian Buffaloes

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	WANG Saiqiao, ZHAO Lu, ZHAI Zhenhan, ZHANG Binglei, JIA Wanhang, WANG Yuqin. Tissue Expression and Bioinformatics Analysis of miR-449a/b Precursor Sequences in Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2175-2184.
[2]	WEI Mingbang, BIANBA Qiongda, XIAO Qingqing, DUAN Mengqi, CHAMBA Yangzom, SHANG Peng. Cloning,Bioinformatics and Expression Analysis of CDKN1B Gene in Tibetan Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2196-2206.
[3]	SUN Chen, GE Sheng, NING Xiaqing, WANG Jiaqi, ZHANG Nuannuan, WANG Xiaoran, ZHANG Shixia. Oxidative Stress Model of BRL-3A Cells Induced by Sodium Hydrosulfite [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2217-2223.
[4]	WANG Jing, SONG Hongbing, WU Yanling, LIN Weimin, XIAO Tianfang. Research Progress of Crocin in Animal Antioxidants [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2233-2244.
[5]	LI Rui, ZHANG Yiyang, LUO Xuehui, ZHOU Manlin, WU Shaomi, YANG Kun, QIAO Zilin. Effect of Hypoxia on Proliferation and Oxidative Stress Injury of Pulmonary Artery Smooth Muscle Cells in Yaks [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2245-2254.
[6]	CHEN Cui, GONG Lei, XU Zhe, WANG Xiaobo. Study on the Inhibition of Hydrogen Peroxide-induced Cellular Aging by AMPK [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2255-2264.
[7]	XU Yuanyuan, ZHENG Haiying, YANG Chunyan, DENG Tingxian, HUANG Chenqian, FENG Chao, LU Xingrong, DUAN Anqin, MO Xia, MA Xiaoya, SHANG Jianghua. Effects of Inhibition of BMP/Smad Signaling Pathway on the Growth and Steroid Hormone Synthesis of Buffalo Ovarian Granulosa Cells [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2354-2362.
[8]	ZHANG Fangwei, ZHANG Qi, LEI Liangliang, SUN Wusheng, ZHANG Di, ZHANG Yunpeng, ZHANG Jingbo, WANG Xiuquan, ZHANG Jing, ZHANG Shumin. Polymorphism of HBEGF Gene and Its Association with Reproductive Traits in Songliao Black Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2370-2379.
[9]	XIAO Peng, SHANG Jianghua, YANG Chunyan, LI Mengqi, DUAN Anqin, MA Xiaoya, FENG Chao, HUANG Chenqian, ZHANG Bo, ZHOU Jinchen, WEI Kelong, ZHENG Wei, ZHENG Haiying. Effects of Alpha-linolenic Acid on Buffalo Ovarian Granulosa Cells Cultured in vitro [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2380-2387.
[10]	SUN Shouhu, CHEN Zhifei, GUO Zhixuan, YU Ting, WANG Xiuwu, LI Songbei, WEI Wenkang, HE Dongsheng. Study on Codon Usage Bias of N gene of Three Porcine Coronaviruses and Their Host Adaptive Mechanism [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2403-2413.
[11]	LI Yongbin, YANG Huilin, SONG Zihao, ZHANG Yu, YANG Jin, MENG Jia, CUI Xiaozhen, LYU Xiaoling, ZHENG Mingxue, BAI Rui. Cloning and Bioinformatics Analysis of MIF Gene in Eimeria tenella [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2450-2459.
[12]	SHI Qiuying, DUAN Baomin, LI Ying, NIU Dengyun, GENG Chenyang, ZHOU Jiawei, WANG Rui, FENG Jingjing. Analysis of Genetic Variation of S1 Gene of Porcine Epidemic Diarrhea Virus in Some Areas of China from 2020 to 2021 [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2468-2478.
[13]	OU Yunwen, PAN Qin, WANG Yang, DAI Junfei, REN Shaoke, ZHANG Yang, ZHANG Jie. Truncated Expression of ORF2 Gene of Porcine Parvovirus Type 6 and Preparation of Its Polyclonal Antibody [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2487-2495.
[14]	CHEN Jianshi, ZHANG Ning, PU Fangxiong, MIAO Jianjia, ZENG Nan, LI Bingxue. Prediction of Bacillus anthracis Carrying Prophages and Analysis of Drug Resistance and Virulence [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2573-2584.
[15]	ZHANG Dianqi, MA Xinhao, YANG Zhimei, MEI Chugang, ZAN Linsen. Bioinformatics Analysis of PAI1 Gene and Its Effect on the Proliferation and Differentiation of Intramuscular Adipocytes in Qinchuan Beef Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1729-1741.