SLT-Ⅱe对PIMVECs分泌炎症反应相关细胞因子的影响

doi:10.16431/j.cnki.1671-7236.2020.11.007

Abstract

Abstract: The aim of the study was to investigate the changes of TNF-α,P-selectin,ICAM-1,NO and ET in porcine intestinal microvascular endothelial cells (PIMVECs) treated with Escherichia coli Shiga-like toxin type Ⅱ variant (SLT-Ⅱe).SLT-Ⅱe was extracted from Escherichia coli by using ultracentrifugation,and the concentration was determined by BAC kit.The purity of SLT-Ⅱe was detected by SDS-PAGE and Western blotting.The optimal incubation time (0,2,4,8,12,24 h) and concentration of SLT-Ⅱe (0.1,0.5,1,5,10 μg/mL) on PIMVECs were evaluated by WST-1.The levels of TNF-α,P-selectin,ICAM-1,NO and ET in cell culture supernatant of control group (0 μg/mL SLT-Ⅱe) and test group (1 μg/mL SLT-Ⅱe) were detected by ELISA.The results showed that using ultracentrifugation could extract pure SLT-Ⅱe with a concentration of 721.75 μg/mL.1 μg/mL SLT-Ⅱe could significantly reduce the activity of PIMVECs after 8 h (P<0.01) and significantly increase the TNF-α secretion of PIMVECs after 9 and 12 h treatment (P<0.05).The NO/ET values at 9 and 12 h were significantly higher than that in the control group (P<0.05).After 9 and 12 h treatment the content of P-selectin was significantly increased (P<0.05) and the levels of ICAM-1 was significantly increased at 12 h (P<0.05).In summary,1 μg/mL SLT-Ⅱe could induce the increase of TNF-α,P-selectin,ICAM-1 and NO/ET secreted by PIMVECs,which laid a foundation for studying the pathogenesis of piglet edema disease.

Key words: Shiga-like toxin type Ⅱ variant; microvascular endothelial cells; inflammatory cytokines; pigs

CLC Number:

WANG Weiran, SHI Xiaojie, ZHANG Meng, MU Xiang. Effects of SLT-Ⅱe on Cytokines of Inflammatory Response Secreted by PIMVECs[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(11): 3467-3473.

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Effects of SLT-Ⅱe on Cytokines of Inflammatory Response Secreted by PIMVECs

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