猪圆环病毒2型江西株的全基因组克隆和序列分析

doi:10.16431/j.cnki.1671-7236.2017.03.024

Abstract

Abstract:

In order to understand the epidemiology and evolution of PCV-2 in Jiangxi province, a pair of primers was designed according to the PCV-2 gene sequence published in GenBank. After PCR amplification, we got the whole genome sequence of 9 strains PCV-2 isolates, and the nucleotide and protein sequences were analyzed, the genetic evolutionary tree was constructed. The results showed that the complete genome of 8 out of the 9 strains were 1 767 bp in length and one strain was 1 768 bp. By analyzing the whole genome sequences of the nucleotide,the homology of nucleotide sequences of the 9 strains was 94.7% to 99.9%.Compared with other whole genome sequences of PCV-2 in GenBank, the homology was 94.3% to 99.8%. The homology of nucleotide sequences of the ORF1 of the 9 strain was 96.9% to 100.0%. ORF2 and ORF3 encoding protein amino acid sequence had some locus mutation. Phylogenetic tree analysis showed that the 9 strains could be divided into 3 genotypes,5 strains belonged to PCV-2b, 3 strains belonged to PCV-2d, and 1 strain belonged to PCV-2a. This study was helpful to monitor and control of PCV-2 in Jiangxi province.

Key words: PCV-2; whole genome; sequence analysis

CLC Number:

S852.65

CAI Gao-feng, LI Kai, ZHANG Li, WANG Wei-song, HE Hou-jun. Cloning and Sequence Analysis of Complete Genomes of Porcine Circovirus Type 2 (PCV-2) Jiangxi Strains[J]. , 2017, 44(3): 790-798.

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Cloning and Sequence Analysis of Complete Genomes of Porcine Circovirus Type 2 (PCV-2) Jiangxi Strains

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