鸭甲肝病毒GX株全基因组序列测定及VP1蛋白生物信息学分析

doi:10.16431/j.cnki.1671-7236.2015.05.001

Abstract

Abstract: In order to investigate the genotypic characteristics and biological characteristics of major capsid protein (VP1) of duck hepatitis A virus (DHAV) GX strain, the whole genome of GX strain was sequenced and compared with 3 serotypes reference strains of DHAV by the molecular biology software.The results showed that the full-length genome of DHAV GX strain was 7 800 bp, including 5'UTR (652 bp), 3'UTR (369 bp) and ORF (6 756 bp) encoding 2 251 aa and its coding products were 12 (VP0/VP3/VP1/2A1/2A2/2A3/2B/2C/3A/3B/3C/3D) at least.Sequence analysis showed that DHAV GX strain could be ranked DHAV 3 style.This strain shared the highest homology with DHAV-3 reference strains; Furthermore, GX strain and DHAV-3 FS strain were in the same cluster, might come from the same ancestor.Amino acid segments locating in 195 to 201 and 211 to 221 aa could be larvaceous dominant B-cell linear epitopes.It suggested that VP1 gene could be used as a candidate gene for the development of genetic engineering vaccine of DHAV.

Key words: duck hepatitis A virus; complete genome; sequence analysis; VP1 protein; B cell epitope

CLC Number:

S852.65⁺9.6

HUANG Yun-xiu, LI Chuan-feng, MENG Chun-chun, CHEN Zong-yan, LI Lu, SONG Kai-jie, LIU Guang-qing, WEI Tian-chao. Determination of the Complete Genome of DHAV GX Strain and Bioinformatics Analysis of VP1 Protein[J]. , 2015, 42(5): 1039-1047.

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Determination of the Complete Genome of DHAV GX Strain and Bioinformatics Analysis of VP1 Protein

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