天津及周边地区猪圆环病毒2型流行情况调查及分离鉴定

doi:10.16431/j.cnki.1671-7236.2018.04.024

Abstract

Abstract:

In order to investigate porcine circovirus type 2 (PCV2) infection and molecular biological characteristics from pig farms in Tianjin and its surrounding areas,the PCR method was used to investigate clinically suspected PCV2 infection case and isolate PCV2 using dulac cells,and the ORF2 gene of PCV2 were cloning,sequencing,and comparing with ORF2 gene of the PCV2 reference strains published in GenBank.The results showed that the positive rate of PCV2 was 25.0% to 38.1% in different regions,the positive rate in summer was obviously higher than that in the other three seasen,reaching 44.0%,and the positive rate of fattening pig was the highest,while that of milking sows was the lowest among different ages pigs.24 strains PCV2 were isolated successfully,the homology of ORF2 sequence was 87.8% to 99.2% with the reference strains,and the homology of 24 isolates strains was 89.4% to 100.0%.The phylogenetic tree analysis showed that 24 isolates belong to two branches.Propagation characteristics of a PCV2 isolate (the Y16155-1 strain) in vitro showed that the virus DNA could be detected at 6 h post inoculation and its numbers reached peak at 72 h post inoculation,the viral titer was 10^-4.3 TCID₅₀/0.2 mL.This result laid a theoretical and scientific foundation for further study molecular epidemiology of PCV2 and the prevention and control of porcine circovirus related diseases in this area.

Key words: Tianjin area; porcine circovirus type 2(PCV2); isolation and identification; sequence analysis

CLC Number:

S852.65⁺9.2

WANG Lili, LU Chao, REN Weike, HAN Wei, LI Fuqiang, ZHENG Li, YAN Minghua. Epidemic Investigation, Isolation and Identification of PCV2 in Tianjin and Its Surrounding Areas[J]. , 2018, 45(4): 1027-1034.

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Epidemic Investigation, Isolation and Identification of PCV2 in Tianjin and Its Surrounding Areas

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