9型猪链球菌广东株的分离鉴定和基因序列分析

doi:10.16431/j.cnki.1671-7236.2018.04.023

Abstract

Abstract:

In order to understand the virulence factor,gene mutation and evolution of Streptococcus suis serotype 9 strain from Guangdong,the cerebrospinal fluid,joint fluid,spleen,liver,lung and lymph node were collected for bacterium culture and biochemical identification,and PCR method was used to identify its serotype and the partial virulence genes.cps9D,gdh,gapdh and orf2 genes were sequenced and analyzed,and phylogenetic trees were constructed.The results showed that the isolate was gram-positive chain cocci,β-hemolysis in blood agar plate,and could ferment most sugars,and amplify cps9D gene successfully,including important conservative gene gdh and virulence genes gapdh and orf2,which proved that it was Streptococcus suis serotype 9.The nucleotide homologies of cps9D,gdh,gapdh and orf2 genes of the isolate with different strains at home and abroad in GenBank were 95.9% to 100.0%,98.6% to 99.8%,98.7% to 99.6% and 95.5% to 99.9%,respectively.It showed that the isolate had higher nucleotide homology with other Streptococcus suis serotype 9 strains at home and abroad,which was basically consistent with the epidemic strains at home and abroad,and there was not much variation.Phylogenetic tree showed that the isolate had high homology and close relationship with Streptococcus suis isolates from different sources at home and abroad,and they were closely related.

Key words: Streptococcus suis serotype 9; isolation and identification; virulence gene; sequence analysis

CLC Number:

S852.65

LI Li, HUANG Liangzong, XIE Bo, ZHANG Hailong, GU Wanjun. Isolation, Identification and Gene Sequence Analysis of Streptococcus suis Serotype 9 Strain from Guangdong[J]. , 2018, 45(4): 1016-1026.

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Isolation, Identification and Gene Sequence Analysis of Streptococcus suis Serotype 9 Strain from Guangdong

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