中国荷斯坦奶牛EEF1D基因的SNPs检测及其与产奶性状的关联分析

doi:10.16431/j.cnki.1671-7236.2018.04.016

Abstract

Abstract:

This study was aimed to investigate the SNP of eukaryotic translation elongation factor 1 delta (EEF1D) gene in Chinese Horstein cow and it's correlation analysis with milk production traits.Sequenom MassARRAY SNP genotyping method was applied to detect the polymorphisms of EEF1D gene in 1 252 Chinese Holstein cows,and the association of EEF1D genotype and genotype combination with milk production traits were analyzed.The results showed that two SNPs were identified,including EEF1D-1 and EEF1D-3 in 5'-flanking region.Two genotypes were found in EEF1D-1 and three genotypes were found in EEF1D-3.Chi-square test showed that EEF1D-1 locus deviated from Hardy-Weinberg equilibrium in Chinese Holstein cow (P < 0.05),but EEF1D-3 locus did not deviate from Hardy-Weinberg equilibrium in Chinese Holstein cow (P > 0.05).Polymorphic information content (PIC) in EEF1D-1 and EEF1D-3 loci were 0.10 and 0.28,showing low polymorphism and moderate polymorphism.In the EEF1D-1 locus,it had extremely significant and significant effect on milk fat percentage and milk protein percentage (P < 0.01) and 305 d milk yield traits (P < 0.05),respectively.In the EEF1D-3 locus,it had extremely significant effects on 305 d milk yield traits,milk fat percentage and milk protein percentage (P < 0.01).The GG-AG and GG-GG genotypes combination in Chinese Holstein cow had significantly greater milk fat percentage than those with GG-AA (P < 0.05).Therefore,EEF1D gene might be useful as molecular markers for milk yield traits in Chinese Holstein cow breeding.

Key words: Chinese Holstein cow; EEF1D gene; milk yield traits; SNP; genotype combination

CLC Number:

S813.3

ZHANG Juan, MU Tong, CAI Zhengyun, GU Yaling, LIU Liyuan, YANG Xueyao, WEN Wan, SUN Dongxiao. SNP Detection of EEF1D Gene in Chinese Horstein Cow and Its Correlation Analysis with Milk Production Traits[J]. , 2018, 45(4): 961-969.

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SNP Detection of EEF1D Gene in Chinese Horstein Cow and Its Correlation Analysis with Milk Production Traits

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