China Animal Husbandry and Veterinary Medicine ›› 2019, Vol. 46 ›› Issue (9): 2715-2725.doi: 10.16431/j.cnki.1671-7236.2019.09.027

• Basic Veterinary Medicine • Previous Articles     Next Articles

Isolation,Identification,Detection of ESBLs and AmpC Enzyme Genotypes and Drug Resistance Analysis of Escherichia coli from Chicken

MA Xin, LIU Yifei, WANG Zhiyu, REN Binlin, CHEN Ze, DU Bin, ZHANG Jing   

  1. Institute of Animal Husbandry and Veterinary Science, Shanxi Academy of Agricultural Science, Taiyuan 030032, China
  • Received:2019-03-29 Online:2019-09-20 Published:2019-09-21

Abstract: To understand the genotype of extend spectrum β-lactamase (ESBLs) and AmpC β-lactamase(AmpC) of Escherichia coli isolated from chicken and actuality of antibiotics resistance in Shanxi province,68 isolated strains accorded with biological traits of chicken Escherichia coli were identified from epidemic materials possessed typical clinical symptoms of chicken colibacillosis.These isolated strains were subjected to K-B disc diffusion and double-disc synergy confirmatory methods recommended by CLSI for drug sensitivity test and resistant phenotype;Genotype of ESBLs and AmpC were detected by PCR method.The results showed that all strains presented resistance to 22 antibiotics in different degrees.The resistant spectrum of 66 strains,accounting for 97.06%,reached to above seven kinds of antibiotics;The number of ESBLs-positive,AmpC-positive and double positive strains were 57(83.82%),19(27.94%) and 16(23.53%),respectively;Resistant genotypes of ESBLs-positive strains were blaTEM,blaOXA and blaCTX-M-1/9,and the genotype of AmpC-positive strains was blaFOX.The results of this research indicated that all isolated strains presented resistance to a majority of antibiotics,and the ESBLs-or/and AmpC-producing strains had been prevalent widely.Genotypes of ESBLs prevalent in Shanxi province were in accordance with those in other regions of China,whereas the blaFOX genotype of AmpC detected in our test was relatively less reported in domestic.

Key words: chicken; Escherichia coli; ESBLs; AmpC; drug resistance; genotype

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