沙冬青种子总黄酮活性成分分析及对小鼠免疫功能的影响

doi:10.16431/j.cnki.1671-7236.2019.09.028

Abstract

Abstract: This study was aimed to analyze and determine the main active components of total flavonoids in the seeds of Ammopiptanthus mongolicus by high performance liquid chromatography-mass spectrometry (LC-MS) and high performance liquid chromatography (HPLC),and explore the effects of total flavonoids in the seeds of Ammopiptanthus mongolicus on immune function in mice.The main active components in total flavonoids of the seeds of Ammopiptanthus mongolicus was analyzed by LC-MS,and the content of the main active components was determined by standard substance under the optimized conditions of HPLC.The effects of proliferation of splenic lymphocytes and KCs were detected by MTT method,the contents of IL-2,IL-4,NO and NOS were measured by ELISA Kit,the antibody-producing cells and hemolysin in mice serum were explored by quantitative hemolytic spectrophotometry and HC₅₀ determination.The results showed that the main compounds of total flavonoids from the seeds of Ammopiptanthus mongolicus were manshank,7,3'-dihydroxy-4'-methoxyflavone,glycitein and amentoflavone,among them,the content of manshank was 52.48%,and the content of 7,3'-dihydroxy-4'-methoxyflavone was 11.20%.The total flavonoids from the seeds of Ammopiptanthus mongolicus had obvious promoting effect on the proliferation of splenic lymphocytes and the production of cytokines,especially at 20 μg/mL total flavonoids,the proliferation rate of splenic lymphocytes and the secretion of IL-2 reached 217.62% and 159.661 pg/mL,respectively,which were extremely significantly higher than that of blank control group (P<0.01);At 40 μg/mL total flavonoids,the secretion of IL-4 in splenic lymphocytes reached 149.274 pg/mL,which was extremely significantly higher than that of blank control group (P<0.01).The total flavonoids from the seeds of Ammopiptanthus mongolicus also significantly promoted the proliferation and NO and NOS secretion of KCs cells.At 60 μg/mL total flavonoids,compared with blank control group,the proliferation rate of KCs cells was 67.77%,the secretion of NO and NOS reached 180.106 and 29.942 μmol/L (P<0.01),respectively.In addition,the total flavonoids could increase the contents of antibody-producing cells and hemolysin in mice serum.The results suggested that the main compounds of total flavonoids from the seeds of Ammopiptanthus mongolicus were manshank and 7,3'-dihydroxy-4'-methoxyflavone.The total flavonoids from the seeds of Ammopiptanthus mongolicus could obviously promote the proliferation of immunocompetent cells,the production and release of related immune cytokines and antibody in mice.

Key words: the seeds of Ammopiptanthus mongolicus; total flavonoids; components; lymphocytes; KCs; IL-2; IL-4; NO; NOS; hemolysin

CLC Number:

R392.3

LIU Sheng, ZHANG Jianan, JIA Ning, FANG Mei, DENG Kang, HUANG Weikuan. Analysis of Active Components of Total Flavonoids from the Seeds of Ammopiptanthus mongolicus and Its Effects on Immune Function in Mice[J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(9): 2726-2736.

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Analysis of Active Components of Total Flavonoids from the Seeds of Ammopiptanthus mongolicus and Its Effects on Immune Function in Mice

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